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AB85075

Anti-AP3 antibody

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(1 Publication)

Rabbit Polyclonal AP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AP3D1.

View Alternative Names

PRO0039, AP3D1, AP-3 complex subunit delta-1, AP-3 complex subunit delta, Adaptor-related protein complex 3 subunit delta-1, Delta-adaptin

1 Images
Western blot - Anti-AP3 antibody (AB85075)
  • WB

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Western blot - Anti-AP3 antibody (AB85075)

All lanes:

Western blot - Anti-AP3 antibody (ab85075) at 1/500 dilution

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HeLa cell lysate

Predicted band size: 130 kDa

Observed band size: 130 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AP3D1. The exact immunogen used to generate this antibody is proprietary information.

O14617

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP3 also known as adaptor-related protein complex 3 is a part of the adaptor protein (AP) complex family involved in cargo selection and vesicle formation for intracellular transport. The complex consists of four subunits with a combined molecular mass of approximately 230 kDa. AP3 predominantly expresses in brain lungs and skin tissues. As a cytosolic complex it helps in the trafficking of proteins to intracellular organelles specifically lysosomes and lysosome-related organelles.
Biological function summary

AP3 plays a central role in the targeting and transport of membrane proteins acting as a scaffold assembling different molecular cargo. It is a component of the AP complex involved in vesicular trafficking within the cell. AP3 interacts with the cytoplasmic tails of cargo proteins to mediate the sorting from the trans-Golgi network to endosomal-lysosomal system. Its function ensures proper protein distribution critical for cellular homeostasis and specialized cellular functions.

Pathways

AP3 influences intracellular trafficking pathways essential for protein sorting. It is especially significant in the biogenesis of lysosome-related organelles. This function aligns it closely with other proteins such as AP1 and AP2 showcasing interconnected roles in protein and membrane trafficking systems. In particular AP3 shares pathways with proteins associated with clathrin contributing to the sorting of integral membrane proteins in endosomal-lysosomal traffic.

Mutations or dysfunction of AP3 are connected to Hermansky-Pudlak syndrome type 2 and other related lysosomal storage disorders. Such ailments result from defective protein sorting and trafficking leading to improper organelle formation and function. In the context of these conditions AP3's interaction with lysosomal proteins becomes important for maintaining normal cellular operations. Moreover AP3 shares pathogenic connectivity with proteins like BLOC-1 and VPS33 highlighting the complexities in these hereditary syndromes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Involved in process of CD8+ T-cell and NK cell degranulation (PubMed : 26744459). In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals (By similarity).
See full target information AP3D1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 88:612-27 PubMed24173221

2013

Hepatocyte nuclear factor 4α and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlei Li,Hanfang Jiang,Linbing Qu,Wenxia Yao,Hua Cai,Ling Chen,Tao Peng
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