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AB201227

Anti-AP3M1 antibody [EPR16385]

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(6 Publications)

Rabbit Recombinant Monoclonal AP3M1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

AP-3 complex subunit mu-1, AP-3 adaptor complex mu3A subunit, Adaptor-related protein complex 3 subunit mu-1, Mu-adaptin 3A, Mu3A-adaptin, AP3M1

3 Images
Flow Cytometry (Intracellular) - Anti-AP3M1 antibody [EPR16385] (AB201227)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AP3M1 antibody [EPR16385] (AB201227)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HEK293 (Human embryonic kidney) cells labeling AP3M1 with ab201227 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-AP3M1 antibody [EPR16385] (AB201227)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-AP3M1 antibody [EPR16385] (AB201227)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling AP3M1 with ab201227 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab201227 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Western blot - Anti-AP3M1 antibody [EPR16385] (AB201227)
  • WB

Supplier Data

Western blot - Anti-AP3M1 antibody [EPR16385] (AB201227)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AP3M1 antibody [EPR16385] (ab201227) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney) cell lysate at 20 µg

Lane 3:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Exposure time: 10s

  • Carrier free

    Anti-AP3M1 antibody [EPR16385] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16385

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product（ab201227） shares 78% homologue with AP3M2. (AP-3 complex subunit mu-2). Cross-reactivity with this protein has not been confirmed experimentally.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75995

Anti-AP2M1 antibody [EP2695Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-AP2M1 antibody [EP2695Y] (AB75995)

  • 1
  • 1
Primary Antibodies

AB133739

Anti-MyD88 antibody [EPR590(N)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [EPR590(N)] (AB133739)

  • 5
  • 1
Primary Antibodies

AB128950

Anti-AP2S1 antibody [EPR2697(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

  • 2
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP3M1 also known as AP-3 complex subunit mu is an important component of the adaptor protein 3 (AP-3) complex. It has a molecular weight of approximately 46 kilodaltons. This protein is expressed in various tissues including neuronal and immune cells indicating its wide-reaching roles in cellular functions. AP3M1 participates actively in cargo recognition and vesicle formation playing a substantial role in the transport and sorting of membrane proteins to specialized compartments within cells.
Biological function summary

The AP3M1 protein integrates into the adaptor protein 3 (AP-3) complex which is important for the trafficking of lysosomal membrane proteins. AP3M1 contributes to assembly and stabilization of the AP-3 complex facilitating the recruitment of clathrin and membrane-bound receptors. This indicates the protein's essential role in the maintenance of cellular homeostasis and intracellular protein traffic impacting the delivery of proteins necessary for lysosomal function and integrity.

Pathways

AP3M1 finds importance in the endosomal-lysosomal pathway and is connected to various cellular processes that manage degradation and recycling of cellular materials. This protein collaborates with other AP-3 complex subunits such as AP3B1 to ensure proper lysosome-related organelles biogenesis. Furthermore AP3M1 intersects with the BLOC-1 (biogenesis of lysosomal organelles complex 1) pathway highlighting its involvement in trafficking and organelle organization specifically in melanosomes and platelet dense granules.

AP3M1 has associations with Hermansky-Pudlak syndrome type 2 and immunodeficiencies. Mutations affecting the AP3M1 function can lead to pigmentary anomalies and immunological defects related to improper lysosomal trafficking. The dysfunction of AP3M1 alongside AP3B1 illustrates its critical role in these diseases as both contribute to the effective formation and function of lysosome-related organelles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
See full target information AP3M1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 221: PubMed36169639

2022

Type II phosphatidylinositol 4-kinases function sequentially in cargo delivery from early endosomes to melanosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyao Zhu,Shuixing Li,Alexa Jaume,Riddhi Atul Jani,Cédric Delevoye,Graça Raposo,Michael S Marks

Life science alliance 5: PubMed34933920

2021

Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Riera-Tur,Tillman Schäfer,Daniel Hornburg,Archana Mishra,Miguel da Silva Padilha,Lorena Fernández-Mosquera,Dennis Feigenbutz,Patrick Auer,Matthias Mann,Wolfgang Baumeister,Rüdiger Klein,Felix Meissner,Nuno Raimundo,Rubén Fernández-Busnadiego,Irina Dudanova

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33990468

2021

AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghyi Kook,Ping Wang,Shufang Meng,Christopher S Jetter,Jennifer M S Sucre,John T Benjamin,Jason J Gokey,Hayley A Hanby,Alexa Jaume,Laura Goetzl,Michael S Marks,Susan H Guttentag

The Journal of cell biology 220: PubMed33886957

2021

A BLOC-1-AP-3 super-complex sorts a cis-SNARE complex into endosome-derived tubular transport carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Shanna L Bowman,Linh Le,Yueyao Zhu,Dawn C Harper,Anand Sitaram,Alexander C Theos,Elena V Sviderskaya,Dorothy C Bennett,Graça Raposo-Benedetti,David J Owen,Megan K Dennis,Michael S Marks

Haematologica 104:2091-2099 PubMed30630984

2019

Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ellie Karampini,Maaike Schillemans,Menno Hofman,Floris van Alphen,Martin de Boer,Taco W Kuijpers,Maartje van den Biggelaar,Jan Voorberg,Ruben Bierings

Cell systems 5:386-398.e4 PubMed29032074

2017

Widespread Post-transcriptional Attenuation of Genomic Copy-Number Variation in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuel Gonçalves,Athanassios Fragoulis,Luz Garcia-Alonso,Thorsten Cramer,Julio Saez-Rodriguez,Pedro Beltrao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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