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Rabbit Recombinant Monoclonal AP3M1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-AP3M1 antibody [EPR16385] - BSA and Azide free (AB251337), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AP3M1 antibody [EPR16385] - BSA and Azide free (AB251337), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-AP3M1 antibody [EPR16385] - BSA and Azide free (AB251337), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

Alternative names

AP-3 complex subunit mu-1, AP-3 adaptor complex mu3A subunit, Adaptor-related protein complex 3 subunit mu-1, Mu-adaptin 3A, Mu3A-adaptin, AP3M1

Recommended products

Rabbit Recombinant Monoclonal AP3M1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16385
Purification technique
Affinity purification Protein A
Specificity

The immunogen used for this product（ab251337） shares 78% homologue with AP3M2. (AP-3 complex subunit mu-2). Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251337 is the carrier-free version of Anti-AP3M1 antibody [EPR16385] ab201227.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AP3M1 also known as AP-3 complex subunit mu is an important component of the adaptor protein 3 (AP-3) complex. It has a molecular weight of approximately 46 kilodaltons. This protein is expressed in various tissues including neuronal and immune cells indicating its wide-reaching roles in cellular functions. AP3M1 participates actively in cargo recognition and vesicle formation playing a substantial role in the transport and sorting of membrane proteins to specialized compartments within cells.

Biological function summary

The AP3M1 protein integrates into the adaptor protein 3 (AP-3) complex which is important for the trafficking of lysosomal membrane proteins. AP3M1 contributes to assembly and stabilization of the AP-3 complex facilitating the recruitment of clathrin and membrane-bound receptors. This indicates the protein's essential role in the maintenance of cellular homeostasis and intracellular protein traffic impacting the delivery of proteins necessary for lysosomal function and integrity.

Pathways

AP3M1 finds importance in the endosomal-lysosomal pathway and is connected to various cellular processes that manage degradation and recycling of cellular materials. This protein collaborates with other AP-3 complex subunits such as AP3B1 to ensure proper lysosome-related organelles biogenesis. Furthermore AP3M1 intersects with the BLOC-1 (biogenesis of lysosomal organelles complex 1) pathway highlighting its involvement in trafficking and organelle organization specifically in melanosomes and platelet dense granules.

Associated diseases and disorders

AP3M1 has associations with Hermansky-Pudlak syndrome type 2 and immunodeficiencies. Mutations affecting the AP3M1 function can lead to pigmentary anomalies and immunological defects related to improper lysosomal trafficking. The dysfunction of AP3M1 alongside AP3B1 illustrates its critical role in these diseases as both contribute to the effective formation and function of lysosome-related organelles.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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