Rabbit Recombinant Monoclonal AP4B1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (AB316003), expandable thumbnail](https://content.abcam.com/products/images/ap4b1-antibody-epr28048-85-bsa-and-azide-free-ab316003--western-blot-img425889.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (AB316003)")
![Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (AB316003), expandable thumbnail](https://content.abcam.com/products/images/ap4b1-antibody-epr28048-85-bsa-and-azide-free-ab316003--western-blot-img425888.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (AB316003)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed:10066790, PubMed:10436028). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (Probable).
Alternative names
AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1, Beta subunit of AP-4, Beta4-adaptin, AP4B1
Recommended products
Rabbit Recombinant Monoclonal AP4B1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28048-85
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316003 is the carrier-free version of Anti-AP4B1 antibody [EPR28048-85] ab316002.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AP4B1 also known as Adaptor-Related Protein Complex 4 Beta 1 Subunit plays a critical role in the cellular transport mechanism. It is a component of the AP-4 adaptor complex which is involved in the sorting of cargo proteins at the trans-Golgi network. The protein has a mass of approximately 82 kDa. AP4B1 is primarily expressed in the brain and other tissues indicating its essential role in neuronal function and development.
- Biological function summary
AP4B1 contributes to the function of the adaptor protein complex 4 (AP-4). This complex is important for recognizing and binding to sorting signals within the cytoplasmic tails of membrane proteins. AP4B1 facilitates the proper trafficking of proteins to endosomes and lysosomes impacting cellular homeostasis and function. Failure in such trafficking processes often leads to cellular malfunction.
- Pathways
The AP4B1 protein is integral to pathways involving vesicular transport and protein sorting. It interacts with other adaptor-related proteins like AP4M1 within the AP-4 complex influencing the export of proteins from the Golgi apparatus. Additionally AP4B1 affects the lysosomal delivery of proteins playing a role in clathrin-independent pathways essential for maintaining cellular stability.
- Associated diseases and disorders
AP4B1 mutations are associated with neurologic conditions such as spastic paraplegia type 47 (SPG47). These mutations lead to improper sorting and trafficking of proteins contributing to neurodegeneration. Furthermore AP4B1 interactions with other proteins in the AP-4 complex like AP4E1 can exacerbate these conditions highlighting the importance of the complex in maintaining neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (ab316003), expandable thumbnail](https://content.abcam.com/products/images/ap4b1-antibody-epr28048-85-bsa-and-azide-free-ab316003--western-blot-img425889.jpg "Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (ab316003)")
Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (ab316003)
This data was developed using Anti-AP4B1 antibody [EPR28048-85] ab316002, the same antibody clone in a different buffer formulation.
The identity of the bands between 20 kDa and 50 kDa are unknown.
Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and Lanes 3-8 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000.
Exposure time: Lanes 1-7: 158 seconds; Lane 8: 180 seconds.
All lanes: Western blot - Anti-AP4B1 antibody [EPR28048-85] (Anti-AP4B1 antibody [EPR28048-85] ab316002) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 6: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 7: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryLanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 3 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 83 kDa
![Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (ab316003), expandable thumbnail](https://content.abcam.com/products/images/ap4b1-antibody-epr28048-85-bsa-and-azide-free-ab316003--western-blot-img425888.jpg "Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (ab316003)")
Western blot - Anti-AP4B1 antibody [EPR28048-85] - BSA and Azide free (ab316003)
This data was developed using Anti-AP4B1 antibody [EPR28048-85] ab316002, the same antibody clone in a different buffer formulation.
Low expression: JEG-3, T84.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 70 seconds; Lanes 2-4: 59 seconds; Lanes 5-6: 70 seconds.
All lanes: Western blot - Anti-AP4B1 antibody [EPR28048-85] (Anti-AP4B1 antibody [EPR28048-85] ab316002) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: T84 (human colon epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 5: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 6: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 83 kDa, 36 kDa
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