Rabbit Polyclonal AP4M1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AP4M1 aa 50-300.
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Publications
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- Journal of cell science 130:2843-28532017Lysosomal targeting of SIDT2 via multiple YxxΦ motifs is required for SIDT2 function in the process of RNautophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViorica Raluca Contu et. al.PubMed 28724756
- Traffic (Copenhagen, Denmark) 18:159-1752017Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b- and AP4-dependent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Hong Toh,Jing Zhi A Tan,Khalisah L Zulkefli,Fiona J Houghton,Paul A GleesonPubMed 28000370
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AP4M1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link O00189
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed:10066790, PubMed:10436028, PubMed:11139587, PubMed:11802162, PubMed:20230749). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system (PubMed:11139587, PubMed:20230749). It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (By similarity). Within AP-4, the mu-type subunit AP4M1 is directly involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos (PubMed:10436028, PubMed:11139587, PubMed:20230749, PubMed:26544806). The adaptor protein complex 4 (AP-4) may also recognize other types of sorting signal (By similarity).
Alternative names
MUARP2, AP4M1, AP-4 complex subunit mu-1, AP-4 adaptor complex mu subunit, Adaptor-related protein complex 4 subunit mu-1, Mu subunit of AP-4, Mu-adaptin-related protein 2, Mu4-adaptin, mu-ARP2, mu4
Recommended products
Rabbit Polyclonal AP4M1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AP4M1 aa 50-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AP4M1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link O00189
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AP4M1 also known as Adaptor Protein Complex 4 Mu Subunit is a component of the adaptor protein complex 4 (AP-4). This protein weighs approximately 50 kDa and functions as a part of protein sorting machinery. It plays a significant mechanical role in vesicular trafficking especially in the selective transport of proteins in the cell. In terms of expression AP4M1 is found in various tissues particularly high levels in brain and kidney tissues indicating its broad involvement in cellular processes.
- Biological function summary
AP4M1 is involved in forming clathrin-coated vesicles which are essential for intracellular transport. As part of the AP-4 complex it interacts with other subunits such as beta4 delta4 and sigma4 contributing to the assembly and specificity required for cargo selection and membrane budding. AP4M1 facilitates the targeting of proteins from the trans-Golgi network (TGN) to endosomes and lysosomes influencing cellular organization and maintenance.
- Pathways
AP4M1 plays an important part in the endocytosis and lysosomal trafficking pathways. It works in coordination with other proteins such as clathrin to mediate the transport and sorting of membrane proteins. The activity of AP4M1 is linked to the sorting of lysosomal enzymes wherein its function ensures proper cellular distribution impacting the efficiency of protein recycling and degradation processes.
- Associated diseases and disorders
AP4M1 mutations have associations with certain neurologic conditions. Mutations in this protein link to spastic paraplegia 50 (SPG50) a neurodegenerative disorder that affects movement. AP4M1's interaction with other AP-4 complex proteins is disturbed in this condition demonstrating its role in neural function. Moreover its contribution to lysosomal trafficking also suggests potential involvement in lysosomal storage disorders where defective trafficking leads to cellular dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-AP4M1 antibody (ab96306)
10% SDS PAGE
All lanes: Western blot - Anti-AP4M1 antibody (ab96306) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 50 kDa
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