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Rabbit Recombinant Monoclonal AP4M1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-AP4M1 antibody [EPR28050-10] (AB323641), expandable thumbnail
  • Western blot - Anti-AP4M1 antibody [EPR28050-10] (AB323641), expandable thumbnail
  • Western blot - Anti-AP4M1 antibody [EPR28050-10] (AB323641), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Mouse
Tested
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways (PubMed:10066790, PubMed:10436028, PubMed:11139587, PubMed:11802162, PubMed:20230749). AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system (PubMed:11139587, PubMed:20230749). It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (By similarity). Within AP-4, the mu-type subunit AP4M1 is directly involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos (PubMed:10436028, PubMed:11139587, PubMed:20230749, PubMed:26544806). The adaptor protein complex 4 (AP-4) may also recognize other types of sorting signal (By similarity).

Additional Targets

Ap4m1, Ap4m1

Alternative names

MUARP2, AP4M1, AP-4 complex subunit mu-1, AP-4 adaptor complex mu subunit, Adaptor-related protein complex 4 subunit mu-1, Mu subunit of AP-4, Mu-adaptin-related protein 2, Mu4-adaptin, mu-ARP2, mu4

Rabbit Recombinant Monoclonal AP4M1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28050-10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641), expandable thumbnail

    Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 2-6 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    The identity of the lower MW band at approximately 37 kDa in lane 5 is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 20 µg

    Lane 5: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641), expandable thumbnail

    Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 2-7 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 1 is incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

    Lanes 6-7 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-5: 180 seconds; Lanes 6-7: 81 seconds.

    All lanes: Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Mouse adrenal gland tissue lysate at 20 µg

    Lane 4: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 5: Rat testis tissue lysate at 20 µg

    Lane 6: Rat adrenal gland tissue lysate at 20 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma cell)whole cell lysate at 20 µg

    Secondary

    Lane 1: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 2 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 36 kDa

  • Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641), expandable thumbnail

    Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 25 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-AP4M1 antibody [EPR28050-10] (ab323641) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting AP4M1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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