AB2000

Anti-APAF1 antibody

(2 Reviews)

(14 Publications)

Rabbit Polyclonal APAF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human APAF1 aa 1150-1200.

View Alternative Names

KIAA0413, APAF1, Apoptotic protease-activating factor 1, APAF-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APAF1 antibody (AB2000)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APAF1 antibody (AB2000)

Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.

Unknown

Western blot - Anti-APAF1 antibody (AB2000)

All lanes:

Western blot - Anti-APAF1 antibody (ab2000) at 1 µg/mL

Lane 1:

Human heart tissue lysate with absence of blocking peptide

Lane 2:

Human heart tissue lysate with presence of blocking peptide

Predicted band size: 141 kDa

Observed band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human APAF1 aa 1150-1200. The exact immunogen used to generate this antibody is proprietary information.

O14727

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APAF1 also known as apoptotic protease activating factor 1 plays a mechanical role in apoptosis by forming a complex with other proteins to trigger cell death. The APAF1 protein has a molecular weight of approximately 130 kDa. It is expressed in many tissues but occurs highly in the brain and heart. APAF1 interacts with cytochrome c and procaspase-9 to promote the activation of the initiator caspase cascade.

Biological function summary

APAF1 functions as a central component of the apoptosome a multi-protein complex important for programmed cell death. This process is essential for normal development and cellular homeostasis. In the apoptosome APAF1 recruits and activates procaspase-9 leading to the activation of caspase-3. This cascade assures that cells die in a controlled manner preventing damage to surrounding tissues.

Pathways

APAF1 serves an important function in the intrinsic pathway of apoptosis. It mediates cytochrome c release from mitochondria a step essential for apoptosis initiation. Another important pathway involving APAF1 is the apoptotic signaling pathway. Proteins such as Bcl-2 family members and p53 influence APAF1's role in these pathways by regulating cytochrome c release and apoptosome assembly.

APAF1 is notably associated with neurodegenerative diseases and certain cancers. Dysregulation of APAF1 can lead to undesirable apoptosis contributing to neurodegenerative conditions like Parkinson’s disease. Conversely reduced APAF1 activity can result in tumor cell survival impacting cancer progression. Altered expression of APAF1 affects its interaction with proteins like Bcl-2 which can inhibit apoptosis and promote cancer cell growth.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

See full target information APAF1

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 59: PubMed34859259

2021

Potential of antiviral drug oseltamivir for the treatment of liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Ju Huang,Chun-Ching Chiu,Min-Hua Hsiao,Jia Le Yow,Bor-Show Tzang,Tsai-Ching Hsu

Head & face medicine 17:37 PubMed34479596

2021

Influences of cold atmospheric plasma on apoptosis related molecules in osteoblast-like cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Benedikt Eggers,Jana Marciniak,Svenja Memmert,Gunar Wagner,James Deschner,Franz-Josef Kramer,Marjan Nokhbehsaim

International journal of oncology : PubMed33469684

2021

Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Gui,Chao Zhang,Xiaomei Xiong,Jing Huang,Juan Xi,Ling Gong,Bisheng Huang,Xiuqiao Zhang

Folia neuropathologica 58:265-274 PubMed33099296

2020

Inhibition of NOX2 contributes to the therapeutic effect of aloin on traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Haihai Dong,Haitao Wang,Xuezhi Zhang

Frontiers in oncology 10:558414 PubMed33178586

2020

The Anti-tumor Effects of Coumaric Acid on Melanoma A375 and B16 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Hu,Zihui Yang,Wenjing Liu,Zhaohai Pan,Xin Zhang,Minjing Li,Xiaona Liu,Qiusheng Zheng,Defang Li

Molecular medicine reports 22:1793-1802 PubMed32582989

2020

Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuli Shao,Shanshan Li,Chang Liu,Weiwei Zhang,Zhenzhu Zhang,Shaowei Zhu,Yunjianan Feng,Yang Pan

Drug development and industrial pharmacy 46:1150-1162 PubMed32482115

2020

The effects of umbilical cord-derived macrophage exosomes loaded with cisplatin on the growth and drug resistance of ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Zhang,Li Liu,Meiling Tang,Hong Li,Xiaoqing Guo,Xiaoqian Yang

RSC advances 9:39082-39089 PubMed35540656

2019

New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Li,Rong Ding,Hao Gao,Liangdong Guo,Xinsheng Yao,Youwei Zhang,Jinshan Tang

Frontiers in oncology 9:853 PubMed31552178

2019

Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohai Pan,Xin Zhang,Pengfei Yu,Xiaoyu Chen,Peng Lu,Minjing Li,Xiaona Liu,Zhipeng Li,Fei Wei,Kejun Wang,Qiusheng Zheng,Defang Li

Molecular therapy. Nucleic acids 16:105-117 PubMed30861413

2019

Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Qing Wang,Xiang-Yu Yang,Xin-Feng Yu,Shu-Xiang Cui,Xian-Jun Qu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APAF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APAF1 antibody (AB2000)

Western blot - Anti-APAF1 antibody (AB2000)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise