Anti-APAF1 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APAF1 antibody (AB2001)

ab2001 at 1μg/ml staining Apaf1 in human heart tissue by IHC

• WB

Lab

Western blot - Anti-APAF1 antibody (AB2001)

Western blot : Anti-APAF1 antibody (ab2001) staining at 1/1500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab2001 was shown to bind specifically to APAF1. A band was observed at 140 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in APAF1 knockout cell line. To generate this image, wild-type and APAF1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-APAF1 antibody (ab2001) at 1/1500 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

APAF1 knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

APAF1 knockout HAP1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

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• WB

Supplier Data

Western blot - Anti-APAF1 antibody (AB2001)

Lane 1:

Western blot - Anti-APAF1 antibody (ab2001) at 1 µg/mL

Lane 2:

Western blot - Anti-APAF1 antibody (ab2001) at 2 µg/mL

All lanes:

K562 cell lysates

Predicted band size: 141 kDa

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