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AB46899

Anti-APAF1 antibody

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(1 Publication)

Rabbit Polyclonal APAF1 antibody. Suitable for IHC-FoFr and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0413, APAF1, Apoptotic protease-activating factor 1, APAF-1

1 Images
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-APAF1 antibody (AB46899)
  • IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-APAF1 antibody (AB46899)

Human testis stained with ab46899 using the antibody at a dilution of 1 : 200

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-FoFr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/200", "IHCFoFr-species-notes": "<p><div>Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.</div>" }, "Mouse": { "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Rat": { "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APAF1 also known as apoptotic protease activating factor 1 plays a mechanical role in apoptosis by forming a complex with other proteins to trigger cell death. The APAF1 protein has a molecular weight of approximately 130 kDa. It is expressed in many tissues but occurs highly in the brain and heart. APAF1 interacts with cytochrome c and procaspase-9 to promote the activation of the initiator caspase cascade.
Biological function summary

APAF1 functions as a central component of the apoptosome a multi-protein complex important for programmed cell death. This process is essential for normal development and cellular homeostasis. In the apoptosome APAF1 recruits and activates procaspase-9 leading to the activation of caspase-3. This cascade assures that cells die in a controlled manner preventing damage to surrounding tissues.

Pathways

APAF1 serves an important function in the intrinsic pathway of apoptosis. It mediates cytochrome c release from mitochondria a step essential for apoptosis initiation. Another important pathway involving APAF1 is the apoptotic signaling pathway. Proteins such as Bcl-2 family members and p53 influence APAF1's role in these pathways by regulating cytochrome c release and apoptosome assembly.

APAF1 is notably associated with neurodegenerative diseases and certain cancers. Dysregulation of APAF1 can lead to undesirable apoptosis contributing to neurodegenerative conditions like Parkinson’s disease. Conversely reduced APAF1 activity can result in tumor cell survival impacting cancer progression. Altered expression of APAF1 affects its interaction with proteins like Bcl-2 which can inhibit apoptosis and promote cancer cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
See full target information APAF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113211 PubMed40831747

2025

Lymphocyte subset-based non-invasive biomarker predicts immunochemotherapy efficacy in EGFR-TKI-pretreated EGFR-mutated NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Lianxi Song,Liang Zeng,Qinqin Xu,Yizhi Li,Wenhuan Guo,Shaoding Lin,Wenjuan Jiang,Zhan Wang,Li Deng,Zhe Huang,Haoyue Qin,Huan Yan,Xing Zhang,Fan Tong,Ruiguang Zhang,Zhaoyi Liu,Lin Zhang,Juan Yu,Xue Yang,Yang Xia,Xiaorong Dong,Gao Zhang,Nong Yang,Yongchang Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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