Mouse Monoclonal 1433B phospho S184 + S186 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YWHAB phospho S184 + S186.
Images
![Flow Cytometry (Intracellular) - APC Anti-14-3-3 beta antibody [03] (AB275731), expandable thumbnail](https://content.abcam.com/products/images/apc-14-3-3-beta-antibody-03-ab275731--flow-cytometry-intracellular-img88260.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - APC Anti-14-3-3 beta antibody [03] (AB275731)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human YWHAB phospho S184 + S186. Database link P31946
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 5 μl/Test |
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Alternative names
14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB
Recommended products
Mouse Monoclonal 1433B phospho S184 + S186 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YWHAB phospho S184 + S186.
Alternative names
14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human YWHAB phospho S184 + S186. Database link P31946
- Clone number
03
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
14-3-3 beta also known as YWHAB is a protein that electrically neutralizes and stabilizes partner proteins. It has a mass of about 30 kDa and is part of the highly conserved 14-3-3 protein family. This family is ubiquitous in eukaryotic cells with 14-3-3 beta being expressed in many tissues including the brain and muscles. Its mechanical role involves binding phosphorylated serine and threonine residues which influences the conformation and function of the target protein.
- Biological function summary
14-3-3 beta plays a central role in cellular processes like signal transduction apoptosis and cell cycle control. It often acts in concert with other proteins forming complexes that regulate these vital functions. For instance it binds to Raf-1 kinase affecting cell growth and differentiation. It also interacts with proteins involved in the mitogen-activated protein kinase (MAPK) pathway highlighting its diverse biological significance.
- Pathways
14-3-3 beta is important in both the MAPK and apoptotic pathways. In the MAPK pathway it helps regulate the phosphorylation status of proteins involved in cell proliferation. The protein binds to Raf-1 an important component in MAPK signaling impacting cellular responses to growth signals. In the context of apoptosis 14-3-3 beta partners with BAD protein a pro-apoptotic factor sequestering it away from mitochondria this interaction is critical for cell survival by preventing the apoptotic process.
- Associated diseases and disorders
Disruptions in 14-3-3 beta function associate with neurodegenerative diseases and cancer. For example its interaction with proteins like Tau connected to Alzheimer's disease points to its involvement in neurodegeneration. Aberrations in its regulatory roles can also contribute to uncontrolled cell growth linked to cancer. Furthermore its interaction with Bcl-2 family proteins ties it to variations in apoptotic regulation seen in cancerous cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry (Intracellular) - APC Anti-14-3-3 beta antibody [03] (ab275731), expandable thumbnail](https://content.abcam.com/products/images/apc-14-3-3-beta-antibody-03-ab275731--flow-cytometry-intracellular-img88260.jpg "Flow Cytometry (Intracellular) - APC Anti-14-3-3 beta antibody [03] (ab275731)")
Flow Cytometry (Intracellular) - APC Anti-14-3-3 beta antibody [03] (ab275731)
Intracellular Flow Cytometry analysis ofHepG2 (human liver hepatocellular carcinoma cell line) cellslabeling 14-3-3 beta with ab275731 at 5 μl/Test (Black) compared with isotype control (Grey).
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