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AB223921

APC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

5

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA3, ACTL3, ACTSG, ACTG2, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin

1 Images
Flow Cytometry (Intracellular) - APC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB223921)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB223921)

Overlay histogram showing SV40LT-SMC cells stained with ab223921 (red line). The cells were fixed with 4% formaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Triton X-100 for 15 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223921, 1/5000 dilution) for 30 minutes at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in SV40LT-SMC cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Triton X-100 for 15 minutes used under the same conditions.

  • Carrier free

    Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] - BSA and Azide free

  • Unconjugated

    Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

  • 519 FITC

    FITC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

  • HRP

    HRP Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

  • 578 PE

    PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E184

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.

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Alternative Products

Primary Antibodies

AB202509

Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368]

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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Primary Antibodies

AB8227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle Actin also known as ACTG2 is an important protein in muscle contraction and cellular structure. It is most commonly found in smooth muscle cells and is not typically expressed in striated muscle. This protein is part of the actin family and has a molecular mass of approximately 42 kDa. ACTG2 plays a role in forming microfilaments which are key components of the muscle contractile machinery. In smooth muscle tissues such as those found in the intestines and blood vessels ACTG2 is often detected together with other cytoskeletal and regulatory proteins.
Biological function summary

ACTG2 contributes to muscle contraction by facilitating the interaction between actin and myosin. It is not considered a part of a protein complex but interacts closely with several other proteins involved in contractile functions. Beyond contraction ACTG2 also supports cellular integrity and shape maintenance. In tissues ACTG2 is involved in activities that require dynamic structural reorganization such as during cellular migration and wound healing highlighting its versatility beyond just a contractile agent.

Pathways

ACTG2 participates in the regulation of the smooth muscle contraction pathway influencing muscle tone and responsiveness to stimuli. The actin-myosin interaction where ACTG2 is involved is critical for the contractile process with other proteins like myosin light chain kinase (MLCK) and tropomyosin playing supportive roles. Additionally the integrin signaling pathway is another context where ACTG2 is indirectly involved aiding in the transmission of mechanical signals from the extracellular matrix to the cell.

ACTG2 is associated with conditions such as megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) and various vascular disorders. Mutations in the ACTG2 gene can disrupt normal muscle function leading to defective peristalsis in the gastrointestinal tract as seen in MMIHS. The connection between ACTG2 and myosin regulatory light chains illustrates how changes in its function can impact muscle contractility and lead to disease. In vascular disorders abnormal expression of ACTG2 is often linked to impaired vessel tone and blood flow regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTA2 acetyl E3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35806053

2022

Synthesis and Biological Evaluation of Small Molecules as Potential Anticancer Multitarget Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Pla-López,Raquel Castillo,Rocío Cejudo-Marín,Olaya García-Pedrero,Mariam Bakir-Laso,Eva Falomir,Miguel Carda
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com