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AB320533

APC Anti-Androgen Receptor antibody [SP242] - C-terminal

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Rabbit Recombinant Monoclonal Androgen Receptor antibody - conjugated to APC.

View Alternative Names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

  • Unconjugated

    Anti-Androgen Receptor antibody [SP242] - C-terminal

  • Carrier free

    Anti-Androgen Receptor antibody [SP242] - BSA and Azide free

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Androgen Receptor antibody [SP242] - C-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Androgen Receptor antibody [SP242] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Androgen Receptor antibody [SP242] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Androgen Receptor antibody [SP242] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Androgen Receptor antibody [SP242] - C-terminal

  • 578 PE

    PE Anti-Androgen Receptor antibody [SP242] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP242

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the AR gene functions as a steroid hormone receptor, a type of ligand-activated transcription factor that regulates eukaryotic gene expression and influences cellular proliferation and differentiation in target tissues. Its transcription factor activity can be modulated by coactivator and corepressor proteins, such as ZBTB7A, which recruits NCOR1 and NCOR2 to androgen response elements on target genes, thereby negatively regulating androgen receptor signaling and androgen-induced cell proliferation. The transcription activation can also be down-regulated by NR0B2. The protein is activated, though not phosphorylated, by HIPK3 and ZIPK/DAPK3. Notably, isoforms 3 and 4 of this protein lack the C-terminal ligand-binding domain and may thus constitutively activate transcription of specific gene sets independently of steroid hormones. This supplementary information is collated from multiple sources and compiled automatically.
See full target information AR
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