AB58

Anti-APC antibody [ALi 12-28]

Name: Anti-APC antibody [ALi 12-28] (ab58) | Abcam
Brand: Abcam Limited
SKU: AB58
Price: 525 USD
Availability: InStock
Rating: 2 (2 reviews)

(2 Reviews)

(40 Publications)

Mouse Monoclonal APC antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-450 of APC, fused to APC.

View Alternative Names

DP2.5, APC, Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 2.5

1 Images

Flow Cytometry - Anti-APC antibody [ALi 12-28] (AB58)

Flow Cyt

Unknown

Flow Cytometry - Anti-APC antibody [ALi 12-28] (AB58)

Overlay histogram showing HCT116 cells stained with ab58 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ALi 12-28

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Fusion protein corresponding to aa 1-450 of APC, fused to APC. The exact immunogen used to generate this antibody is proprietary information.

P25054

Epitope

ALI-12-28 was epitope mapped by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test and was found to bind to APC in the region between nucleotides 135 and 422 (exons 2-3) (Efstathiou et al.).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 99.98% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton (PubMed : 17293347). Plays a role in mediating the organization of F-actin into ordered bundles (PubMed : 17293347). Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules (By similarity). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

See full target information APC

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabl5942 PubMed35417240

2022

The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Mastrogiovanni,Pablo Vargas,Thierry Rose,Céline Cuche,Elric Esposito,Marie Juzans,Hélène Laude,Amandine Schneider,Mathilde Bernard,Sophie Goyard,Charlotte Renaudat,Marie-Noëlle Ungeheuer,Jérôme Delon,Andrés Alcover,Vincenzo Di Bartolo

The Biochemical journal 478:2297-2308 PubMed34143865

2021

Inhibition of selective autophagy by members of the herpesvirus ubiquitin-deconjugase family.

Applications

Unspecified application

Species

Unspecified reactive species

Päivi Ylä-Anttila,Maria G Masucci

Nature communications 12:3656 PubMed34135347

2021

Spatial regulation of thermomorphogenesis by HY5 and PIF4 in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghwa Lee,Wenli Wang,Enamul Huq

Nature communications 12:3426 PubMed34103516

2021

Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Friedrich,Vicky Oberkofler,Inês Trindade,Simone Altmann,Krzysztof Brzezinka,Jörn Lämke,Michal Gorka,Christian Kappel,Ewelina Sokolowska,Aleksandra Skirycz,Alexander Graf,Isabel Bäurle

Theranostics 11:7600-7615 PubMed34158869

2021

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Hongmei Zhao,Hongqin Yang,Chi Geng,Yang Chen,Yaqin Tang,Zhiwei Li,Junling Pang,Ting Shu,Yu Nie,Yongshuo Liu,Kegang Jia,Jing Wang

Frontiers in immunology 12:666231 PubMed34149701

2021

Optical Control of CD8 T Cell Metabolism and Effector Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea M Amitrano,Brandon J Berry,Kihong Lim,Kyun-Do Kim,Richard E Waugh,Andrew P Wojtovich,Minsoo Kim

eLife 10: PubMed34075876

2021

Repression by the TOPLESS corepressor requires association with the core mediator complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander R Leydon,Wei Wang,Hardik P Gala,Sabrina Gilmour,Samuel Juarez-Solis,Mollye L Zahler,Joseph E Zemke,Ning Zheng,Jennifer L Nemhauser

iScience 24:102670 PubMed34189435

2021

Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Inbal Wortzel,Galia Maik-Rachline,Suresh Singh Yadav,Tamar Hanoch,Rony Seger

Frontiers in oncology 11:664689 PubMed34094957

2021

Regulation of ERα Stability and Estrogen Signaling in Breast Cancer by HOIL-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jianing Ding,Peng Kuang

Journal of extracellular vesicles 10:e12088 PubMed34025953

2021

WNT3A-loaded exosomes enable cartilage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Bethan L Thomas,Suzanne E Eldridge,Babak Nosrati,Mario Alvarez,Anne-Sophie Thorup,Giovanna Nalesso,Sara Caxaria,Aida Barawi,James G Nicholson,Mauro Perretti,Carles Gaston-Massuet,Costantino Pitzalis,Alison Maloney,Adrian Moore,Ray Jupp,Francesco Dell'Accio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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