JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB305567

APC Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody - conjugated to APC. Suitable for Antibody Labelling, Target Binding Affinity, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1, KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

  • Carrier free

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

  • Unconjugated

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • HRP

    HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 578 PE

    PE Anti-Aspartate Aminotransferase +FABP-1 antibody [EPR12145]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12145

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, Antibody Labelling, Target Binding Affinity, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

style
left-column

Complementary Products

Assay Kits

AB105135

Aspartate Aminotransferase Activity Assay Kit

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

  • 5
  • 1
Assay Kits

AB263882

Mouse AST ELISA Kit (Aspartate Aminotransferase)

Recombinant|SimpleStep

ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

  • 5
  • 1
Primary Antibodies

AB181547

Anti-Insulin antibody [EPR17359]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Insulin antibody [EPR17359] (AB181547)

  • 5
  • 11
Primary Antibodies

AB32412

Anti-C Reactive Protein antibody [Y284]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

  • 5
  • 6
Primary Antibodies

AB48651

Biotin Anti-Creatine kinase B type antibody

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as glutamate oxaloacetate transaminase is an enzyme that plays an important role in amino acid metabolism. AST transfers an amino group typically from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. This enzyme approximately weighs 45 kDa. AST is widely expressed in tissues with especially high levels found in the liver heart skeletal muscle and kidneys.
Biological function summary

Aspartate aminotransferase participates in the malate-aspartate shuttle which facilitates the transfer of reducing equivalents across the mitochondrial membrane. This shuttle system is important in maintaining the balance of NADH and NAD+ between the cytosol and mitochondria. AST forms part of a protein complex that includes α-ketoglutarate and oxaloacetate which are central to its transaminase activity in cellular metabolism.

Pathways

Aspartate aminotransferase is significantly involved in the tricarboxylic acid (TCA) cycle and the urea cycle. Within the TCA cycle AST ensures the linkage between carbohydrate and amino acid metabolism by converting aspartate and α-ketoglutarate into oxaloacetate and glutamate. In the urea cycle the enzyme connects to argininosuccinate synthetase and argininosuccinate lyase facilitating the removal of excess nitrogen from the body.

Elevated levels of aspartate aminotransferase are often indicative of liver damage including conditions like hepatitis and cirrhosis. Additionally the enzyme associates with myocardial infarction due to its high activity in cardiac tissue. Liver-related disorders may also involve fatty acid-binding protein 1 (FABP-1) which closely interacts with AST and serves as a marker for liver function and damage.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).
See full target information Aspartate aminotransferase, cytoplasmic

Additional targets

GOT2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com