Rabbit Recombinant Monoclonal Beta-3-tubulin antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (AB224977), expandable thumbnail](https://content.abcam.com/products/images/apc-beta-iii-tubulin-antibody-ep1569y-neuronal-marker-ab224977--flow-cytometry-intracellular-img26640.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (AB224977)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes The cellular localisation of this product has been verified in ICC/IF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Version
2 products for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Alternative names
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
Recommended products
Rabbit Recombinant Monoclonal Beta-3-tubulin antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP1569Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Do Not Freeze, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224977), expandable thumbnail](https://content.abcam.com/products/images/apc-beta-iii-tubulin-antibody-ep1569y-neuronal-marker-ab224977--flow-cytometry-intracellular-img26640.jpg "Flow Cytometry (Intracellular) - APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224977)")
Flow Cytometry (Intracellular) - APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224977)
Overlay histogram showing U87MG cells stained with ab224977 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224977, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Downloads
Product protocols
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