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Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to APC. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, African green monkey samples.

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Images

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754), expandable thumbnail
  • Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754), expandable thumbnail
  • Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (AB317754), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (AB317754), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Tested
African green monkey
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Species
African green monkey
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
African green monkey
Dilution info
1/500
Notes

-

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

Recommended products

Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to APC. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, African green monkey samples.

Key facts

Isotype
IgG
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16774
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Associated diseases and disorders

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab317754), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab317754)

    Beta Tubulin Flow Cytometry (Intracellular) staining of HeLa (human cervical adenocarcinoma epithelial cell) using rabbit Anti-beta Tubulin antibody

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab317754), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab317754)

    Beta Tubulin Flow Cytometry (Intracellular) staining of NIH/3T3 (mouse embryonic fibroblast) using rabbit Anti-beta Tubulin antibody

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab317754), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab317754)

    Beta Tubulin Flow Cytometry (Intracellular) staining of COS-1 (African green monkey kidney fibroblast-like cell) using rabbit Anti-beta Tubulin antibody

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized COS-1 (African green monkey kidney fibroblast-like cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (ab317754), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (ab317754)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized COS-1 (African green monkey kidney fibroblast-like) cells labelling beta Tubulin with ab317754 at 1/50 (10.0 ug/ml) dilution (Magenta).

    Confocal image showing cytoplasmic staining in COS-1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (ab317754), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (ab317754)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling beta Tubulin with ab317754 at 1/50 (10.0 ug/ml) dilution (Magenta).

    Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (ab317754), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] (ab317754)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling beta Tubulin with ab317754 at 1/50 (10.0 ug/ml) dilution (Magenta).

    Confocal image showing cytoplasmic staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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