AB155356

APC Anti-CCR3 antibody [5E8-G9-B4]

Name: APC Anti-CCR3 antibody [5E8-G9-B4] (ab155356) | Abcam
Brand: Abcam Limited
SKU: AB155356
Price: 370 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CCR3 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CCR3 protein.

View Alternative Names

CD193, CMKBR3, CCR3, C-C chemokine receptor type 3, C C CKR3, C-C CKR-3, CC-CKR-3, CCR-3, CKR 3, CKR3, Eosinophil eotaxin receptor

1 Images

Flow Cytometry - APC Anti-CCR3 antibody [5E8-G9-B4] (AB155356)

Flow Cyt

Unknown

Flow Cytometry - APC Anti-CCR3 antibody [5E8-G9-B4] (AB155356)

Flow cytometric analysis of normal Human peripheral blood cells labeling CCR3 with Anti-Human CD16 PE and mouse IgG2b kappa Isotype Control APC (left) or 5 µl (0.25 µg) of ab155356 (right). Cells in the large scatter population were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E8-G9-B4

Isotype

IgG2b

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CCR3 protein. The exact immunogen used to generate this antibody is proprietary information.

P51677

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/apc-mouse-igg2b-plpv219-isotype-control-ab91534'>ab91534</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR3 also known as C-C chemokine receptor type 3 is a protein that acts as a receptor for chemokines. The molecular weight of CCR3 is approximately 43 kDa. This protein is predominantly expressed in eosinophils and basophils and is also found in specific subsets of T cells. The expression of CCR3 in these cells plays an important role in directing their migration and activity. This receptor is part of the G protein-coupled receptor family which transduce signals across the cell membrane.

Biological function summary

CCR3 facilitates chemokine-directed migration of white blood cells an essential function for immune responses. It binds to several chemokines like eotaxin eotaxin-2 and RANTES which mediates the recruitment of eosinophils and other cells to sites of inflammation. CCR3 does not form part of a protein complex instead it interacts directly with its chemokine ligands. This activity enhances the body's ability to respond to allergic reactions and inflammatory processes.

Pathways

CCR3 plays a role in the signaling pathways that orchestrate immune cell trafficking and activation. It is involved in the chemokine signaling pathway which regulates cell movement and positioning and the calcium mobilization pathway through its ligand interaction. CCR3 along with other receptors like CCR5 and CXCR4 facilitates the communication between immune cells aiding in directed cell movement towards the inflammation site.

CCR3 is notably involved in allergic disorders and asthma. Its expression influences the recruitment of eosinophils which are key players in these conditions. This receptor is linked to heightened inflammatory responses leading to tissue damage in chronic allergic diseases. The interaction of CCR3 with chemokines like eotaxin is significant in the pathophysiology of these diseases making it a potential target for therapeutic intervention to mitigate symptoms of chronic allergic responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G protein-coupled receptor (GPCR) that plays a key role in the immune system by regulating the migration and activation of white blood cells in response to chemokines (PubMed : 28994588). Selectively interacts with eosinophil-attracting chemokines such as eotaxin/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26 (PubMed : 7622448, PubMed : 8642344, PubMed : 8676064). Ligand binding triggers intracellular signaling that leads to chemotaxis of immune cells. Mechanistically, signals through GNA14 or GNA16 to induce stimulation of phospholipase Cbeta/PLCB2 and subsequently chemotaxis (PubMed : 18406577). Alternatively, transduces signal via GNAI1 resulting in elevated intracellular calcium levels and activation of the PI3K/AKT pathway (PubMed : 8676064, PubMed : 35570218). May also act as a possible functional receptor for NARS1 (PubMed : 30171954).. (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.

See full target information CCR3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 118:4753-4761 PubMed28513874

2017

β3-Adrenergic Regulation of EPC Features Through Manipulation of the Bone Marrow MSC Niche.

Applications

Unspecified application

Species

Unspecified reactive species

Rana Vafaei,Seyed Mahdi Nassiri,Vahid Siavashi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CCR3 antibody [5E8-G9-B4]

Flow Cytometry - APC Anti-CCR3 antibody [5E8-G9-B4] (AB155356)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise