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AB326454

APC Anti-CD-10 antibody [MEM-78]

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Mouse Monoclonal CD10 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE

2 Images
Flow Cytometry - APC Anti-CD-10 antibody [MEM-78] (AB326454)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD-10 antibody [MEM-78] (AB326454)

Separation of human neutrophil granulocytes (red-filled) from 10 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326454 (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-CD-10 antibody [MEM-78] (AB326454)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD-10 antibody [MEM-78] (AB326454)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326454 (10 μl reagent / 100 μl of peripheral whole blood).

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD10 antibody [MEM-78]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD10 antibody [MEM-78]

  • 519 FITC

    FITC Anti-CD10 antibody [MEM-78]

  • 578 PE

    PE Anti-CD-10 antibody [MEM-78]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD10 antibody [MEM-78]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD10 antibody [MEM-78]

  • 618 PE/DyLight™ 594

    PE/DyLight™ 594 Anti-CD10 antibody [MEM-78]

  • 675 PerCP

    PerCP Anti-CD10 antibody [MEM-78]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD10 antibody [MEM-78]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-78

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody MEM-78 reacts with an extracellular epitope CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed : 15283675, PubMed : 6208535, PubMed : 6349683, PubMed : 8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed : 17101991, PubMed : 6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed : 6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed : 15283675, PubMed : 6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed : 16254193, PubMed : 2531377, PubMed : 2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).
See full target information MME
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Product promise

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