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AB326452

APC Anti-CD134 / OX40L receptor antibody [1A5]

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Mouse Monoclonal SIGLEC5 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD170, CD33L2, OBBP2, SIGLEC5, Sialic acid-binding Ig-like lectin 5, Siglec-5, CD33 antigen-like 2, Obesity-binding protein 2, OB-BP2, OB-binding protein 2

2 Images
Flow Cytometry - APC Anti-CD134 / OX40L receptor antibody [1A5] (AB326452)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD134 / OX40L receptor antibody [1A5] (AB326452)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326452 (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-CD134 / OX40L receptor antibody [1A5] (AB326452)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD134 / OX40L receptor antibody [1A5] (AB326452)

Separation of human neutrophil granulocytes (red-filled) from CD170 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326452 (10 μl reagent / 100 μl of peripheral whole blood).

  • Unconjugated

    Anti-SIGLEC5 antibody [1A5]

  • 578 PE

    PE Anti-SIGLEC5 antibody [1A5]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A5

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
See full target information SIGLEC5
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Product promise

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