Mouse Monoclonal CD146 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCAM aa 1-600.
Images
![Flow Cytometry - APC Anti-CD146 antibody [01] (AB275616), expandable thumbnail](https://content.abcam.com/products/images/apc-cd146-antibody-01-ab275616--flow-cytometry-img83340.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - APC Anti-CD146 antibody [01] (AB275616)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- APC
- Excitation/Emission
- Ex: 650nm, Em: 660nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human MCAM aa 1-600. The exact immunogen used to generate this antibody is proprietary information. Database link P43121
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use at 5 μL per test |
Target data
Function
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Alternative names
CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
Recommended products
Mouse Monoclonal CD146 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCAM aa 1-600.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- APC
- Excitation/Emission
- Ex: 650nm, Em: 660nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human MCAM aa 1-600. The exact immunogen used to generate this antibody is proprietary information. Database link P43121
- Clone number
- 01
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
- Biological function summary
CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.
- Pathways
CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.
- Associated diseases and disorders
CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - APC Anti-CD146 antibody [01] (ab275616), expandable thumbnail](https://content.abcam.com/products/images/apc-cd146-antibody-01-ab275616--flow-cytometry-img83340.jpg "Flow Cytometry - APC Anti-CD146 antibody [01] (ab275616)")
Flow Cytometry - APC Anti-CD146 antibody [01] (ab275616)
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD146 with ab275616 at 5 μL per test (Black) compared to an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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