AB269325

APC Anti-CD161 antibody [HP-3G10]

Name: Anti-CD161 APC antibody [HP-3G10] (ab269325) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB269325
Price: 140 USD
Availability: InStock

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Mouse Monoclonal KLRB1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing KLRB1 protein.

View Alternative Names

CD161, CLEC5B, NKRP1A, KLRB1, Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, HNKR-P1a, Natural killer cell surface protein P1A, NKR-P1A

1 Images

Flow Cytometry - APC Anti-CD161 antibody [HP-3G10] (AB269325)

Flow Cyt

Unknown

Flow Cytometry - APC Anti-CD161 antibody [HP-3G10] (AB269325)

Flow cytometry analysis (surface staining) of human peripheral blood cells labeling CD161 with ab269325.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HP-3G10

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing KLRB1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q12918

Specificity

ab269325 recognizes an extracellular epitope of CD161.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Or 100 μL of whole blood.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD161 also known as NKRP1A or KLRB1 is a type II transmembrane protein expressed primarily on natural killer (NK) cells and subsets of T cells. Weighing approximately 30-40 kDa CD161 functions as a C-type lectin receptor involved in the immune response. Its expression can be detected in various tissues including the liver and spleen. Researchers utilize tools such as mouse CD161 PE as well as CD161 APC antibodies for study of its cell surface expression and role in immune modulation.

Biological function summary

CD161 plays a role in immune system regulation by mediating NK cell and T cell activity. It does not operate as part of a complex but interacts with other cellular receptors to modulate immune responses. For example it serves as an inhibitory receptor often through engagement with its ligand LLT1 to temper immune responses and prevent overactivation. This balance is key to maintaining immune homeostasis and facilitates pathogen clearance while avoiding excessive tissue damage.

Pathways

CD161 is involved in the immune checkpoint pathways and is linked to inflammatory response signaling. These pathways play an important role in the immune system's ability to discern self from non-self determining the intensity and character of the body's defense mechanisms. Other proteins such as CTLA-4 and PD-1 also function within these pathways contributing to the regulation of immune cell activity and preventing autoimmunity.

CD161 is associated with inflammatory diseases and cancer. Its involvement in immune checkpoints implicates it in conditions such as autoimmune diseases where it may fail to properly regulate immune activity including multiple sclerosis. In cancer CD161 expression on T cells has been examined in the context of its inhibitory role potentially modulating the immune system's ability to target tumor cells effectively. In these scenarios its interaction with LLT1 and other immune checkpoint proteins influences the disease course and therapeutic outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

See full target information KLRB1

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