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AB77230

APC Anti-CD177 antibody [MEM-166]

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(4 Publications)

Mouse Monoclonal CD177 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD177 protein.

View Alternative Names

CD177, NB1, PRV1, UNQ595/PRO1181, CD177 antigen, Human neutrophil alloantigen 2a, NB1 glycoprotein, Polycythemia rubra vera protein 1, HNA-2a, NB1 GP, PRV-1

2 Images
Flow Cytometry - APC Anti-CD177 antibody [MEM-166] (AB77230)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD177 antibody [MEM-166] (AB77230)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab77230 (concentration in sample 0.56 μg/ml).

Flow Cytometry - APC Anti-CD177 antibody [MEM-166] (AB77230)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD177 antibody [MEM-166] (AB77230)

Separation of human CD117 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab77230 (concentration in sample 0.56 µg/ml).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD177 antibody [MEM-166]

  • 519 FITC

    FITC Anti-CD177 antibody [MEM-166]

  • 578 PE

    PE Anti-CD177 antibody [MEM-166]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-166

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD177 protein. The exact immunogen used to generate this antibody is proprietary information.

Q8N6Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD177 also known as neutrophil antigen NB1 is a glycoprotein with an approximate mass of 58 kDa. It is primarily expressed on the surface of neutrophils a type of white blood cell involved in immune response. The expression of CD177 can vary among individuals where some people might have a higher or lower presence on their neutrophils. CD177 has been identified to play a role in cell adhesion and signaling functions contributing to various cellular processes within the immune system.
Biological function summary

CD177 functions by mediating interactions between neutrophils and endothelial cells during inflammation. This protein is not known to be part of a multi-protein complex but it plays a significant role in facilitating neutrophil transmigration from blood vessels to sites of tissue damage or infection. This process supports the body's defense mechanisms by enabling neutrophils to quickly respond to external threats and participate in the inflammatory response.

Pathways

The involvement of CD177 in chemotactic activity and cell migration highlights its contribution to both the immune response and inflammation pathways. Its interaction with proteins such as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) enhances neutrophil transmigration across the endothelium. CD177 acts within these pathways to ensure a precise and rapid movement of neutrophils which is essential for an effective immune response.

CD177 levels have associations with conditions like polycythemia vera and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. In polycythemia vera CD177 can affect the proliferation of blood cells due to its role in neutrophil function. In ANCA-associated vasculitis CD177's interaction with proteins such as PR3 (Proteinase 3) on the surface of neutrophils could contribute to the pathophysiology of the disease leading to inappropriate activation of the immune system and subsequent tissue damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF primed neutrophils including degranulation and superoxide production (PubMed : 21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed : 28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed : 17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed : 23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed : 17244676, PubMed : 18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed : 23202369).
See full target information CD177
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Blood transfusion = Trasfusione del sangue 20:465-474 PubMed35848624

2022

Human neutrophil antigen 3 genotype impacts neutrophil-mediated endothelial cell cytotoxicity in a two-event model of TRALI.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Chiaretti,Mark Burton,Penny Hassel,Filip Radenkovic,Nilam Devikashri,Annette J Sultana,Fergal T Temple,Melinda M Dean,John-Paul Tung

Experimental and therapeutic medicine 15:2525-2532 PubMed29467851

2018

Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Yi Liu,Yi Li,Zhaoyun Liu,Liyan Li,Shaoxue Ding,Yihao Wang,Tian Zhang,Lijuan Li,Zonghong Shao,Rong Fu

American journal of clinical pathology 140:658-69 PubMed24124144

2013

CD177 expression on neutrophils: in search of a clonal assay for myeloid neoplasia by flow cytometry.

Applications

Flow Cyt

Species

Human

Howard J Meyerson,Ebeneezer Osei,Karen Schweitzer,Georgetta Blidaru,Alison Edinger,Anna Balog

The Journal of experimental medicine 207:1951-66 PubMed20733031

2010

CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.

Applications

Flow Cyt

Species

Human

Jing Zhou,Paul C Y Tang,Lingfeng Qin,Peter M Gayed,Wei Li,Eleni A Skokos,Themis R Kyriakides,Jordan S Pober,George Tellides
View all publications
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