AB40914

APC Anti-CD18 antibody [MEM-48]

Name: Anti-CD18 APC antibody [MEM-48] (ab40914) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB40914
Price: 170 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD18 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing ITGB2 protein.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

1 Images

Flow Cytometry - APC Anti-CD18 antibody [MEM-48] (AB40914)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD18 antibody [MEM-48] (AB40914)

Flow cytometry analysis (surface staining) of human peripheral blood using ab40914.

578 PE

PE Anti-CD18 antibody [MEM-48]
519 FITC

FITC Anti-CD18 antibody [MEM-48]
Carrier free

Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free
Unconjugated

Anti-CD18 antibody [MEM-48]
Biotin

Biotin Anti-CD18 antibody [MEM-48]
723 Alexa Fluor® 700

Alexa Fluor® 700 Anti-CD18 antibody [MEM-48]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-48

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ITGB2 protein. The exact immunogen used to generate this antibody is proprietary information.

P05107

Epitope

This antibody recognizes an epitope including amino acids 534-546 in the cysteine-rich repeat 3 of the CD18 antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" } } }

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Product details

The conjugate was purified by size-exclusion chromatography and adjusted for direct use.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.

Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407).

See full target information ITGB2

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Publications (2)

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Journal of immunology (Baltimore, Md. : 1950) 166:5629-37 PubMed11313403

2001

Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

C Lu,M Ferzly,J Takagi,T A Springer

Folia biologica 36:41-50 PubMed1971601

1990

Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Stefanová,I Hilgert,H Kristofová,S Vanĕk,V Horejsí

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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APC Anti-CD18 antibody [MEM-48]

Flow Cytometry - APC Anti-CD18 antibody [MEM-48] (AB40914)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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