APC Anti-CD19 antibody [EPR5906]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - APC Anti-CD19 antibody [EPR5906] (AB317484)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD19 with ab317484 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : Jurkat (PMID : 19147785)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APC Anti-CD19 antibody [EPR5906] (AB317484)

Immunohistochemical analysis of paraffin-embedded Human diffuse large tissue labeling CD19 with ab317484 at 1/100 (5.0 ug/ml) dilution.

Membranous staining on human diffuse large B-cell lymphoma. The section was incubated with ab317484 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - APC Anti-CD19 antibody [EPR5906] (AB317484)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD19 with ab317484 at 1/50 dilution (Magenta).

Confocal image showing membranous staining in Raji cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Negative control : Jurkat (PMID : 19147785) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Green). The Nuclear counterstain was DAPI (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APC Anti-CD19 antibody [EPR5906] (AB317484)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling CD19 with ab317484 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human skeletal muscle. The section was incubated with ab317484 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APC Anti-CD19 antibody [EPR5906] (AB317484)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD19 with ab317484 at 1/100 (5.0 ug/ml) dilution.

Membranous staining on human tonsil.The section was incubated with ab317484 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.