AB18224

APC Anti-CD19 antibody [LT19]

Name: Anti-CD19 APC antibody [LT19] (ab18224) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB18224
Price: 535 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal CD19 antibody - conjugated to APC. Suitable for IP, Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing CD19 protein.

View Alternative Names

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LT19

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, IP

applications

Immunogen

Cell preparation containing CD19 protein. The exact immunogen used to generate this antibody is proprietary information.

P15391

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified by size exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: BSA, PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).

See full target information CD19

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 32:2080-2093 PubMed38715362

2024

Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Smith,Zachary C Elmore,Robert M Fusco,Joshua A Hull,Alan Rosales,Michele Martinez,Alice F Tarantal,Aravind Asokan

Proceedings of the National Academy of Sciences of the United States of America 119:e2120311119 PubMed35482917

2022

An antagonistic pleiotropic gene regulates the reproduction and longevity tradeoff.

Applications

Unspecified application

Species

Unspecified reactive species

Dou Wu,Zi Wang,Jingying Huang,Liang Huang,Songbo Zhang,Ruixue Zhao,Wei Li,Di Chen,Guangshuo Ou

Therapeutic advances in chronic disease 12:20406223211007369 PubMed33995992

2021

MicroRNA-21 from bone marrow mesenchymal stem cell-derived extracellular vesicles targets TET1 to suppress KLF4 and alleviate rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Qing Li,Yu-Xuan Fang,Ying Liu,Fan-Ru Meng,Xia Wu,Chun-Wang Zhang,Yu Zhang,Yan-Qing Liu,Dan Liu

Frontiers in medicine 7:577578 PubMed33425934

2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Jiang,Jun Zhang,Zhengwei Li,Guoliang Jia

Stem cell research & therapy 11:455 PubMed33109266

2020

microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ding,Jinhua Liu,Xiaofei Zhang

PLoS pathogens 9:e1003837 PubMed24385909

2013

Pfit is a structurally novel Crohn's disease-associated superantigen.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Liu,Hui Chen,Matthew B Brecher,Zhong Li,Bo Wei,Bisweswar Nandi,Jing Zhang,Hua Ling,Gary Winslow,Jonathan Braun,Hongmin Li

PloS one 7:e31998 PubMed22384124

2012

Phenotypic detection of clonotypic B cells in multiple myeloma by specific immunoglobulin ligands reveals their rarity in multiple myeloma.

Applications

Flow Cyt

Species

Human

Martin Trepel,Victoria Martens,Christian Doll,Janina Rahlff,Barbara Gösch,Sonja Loges,Mascha Binder

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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APC Anti-CD19 antibody [LT19]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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