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AB326459

APC Anti-CD1A antibody [HI149]

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Mouse Monoclonal CD1A antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD1a, T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

4 Images
Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using ab326459 (concentration in sample 0.5 μg/ml).

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using ab326459 (concentration in sample 0.5 μg/ml) and anti-human CD11c (BU15) PE antibody (20 μl reagent per million cells in 100 μl of cell suspension).

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)

Separation of CD11c positive cells stained using ab326459 (concentration in sample 0.5 μg/ml, red-filled) from CD11c positive cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.5 μg/ml, same as CD1a APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD1A antibody [HI149] (AB326459)

Separation of MOLT-4 cells stained using ab326459 (concentration in sample 4 μg/m, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 4 μg/ml, same as CD1a APC concentration, black-dashed) in flow cytometry analysis (surface staining).

  • 519 FITC

    FITC Anti-CD1a antibody [HI149]

  • 578 PE

    PE Anti-CD1A antibody [HI149]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD1a antibody [HI149]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD1a antibody [HI149]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HI149

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1A
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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