AB234217

APC Anti-CD1a antibody [SK9]

Name: APC Anti-CD1a antibody [SK9] (ab234217) | Abcam
Brand: Abcam Limited
SKU: AB234217
Price: 310 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD1A antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD1A protein.

View Alternative Names

CD1a, T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

2 Images

Flow Cytometry - APC Anti-CD1a antibody [SK9] (AB234217)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD1a antibody [SK9] (AB234217)

Flow cytometry surface staining pattern of human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells stained using ab234217 (10 μL reagent / 100 μL of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD1a antibody [SK9] (AB234217)

Separation of human CD1a positive cells (red-filled) from cells stained using mouse IgG1 isotype control APC antibody (concentration in sample 5 μg/ml, same as CD11a APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells stained using ab234217 (10 μL reagent / 100 μL of peripheral whole blood).

578 PE

PE Anti-CD1a antibody [SK9]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SK9

Isotype

IgG2b

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD1A protein. The exact immunogen used to generate this antibody is proprietary information.

P06126

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μL whole blood)</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab234217 is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1a also known as T6 antigen or CD1 is a protein with an estimated molecular mass of 37 kDa. It is expressed mainly on the surface of Langerhans cells in the skin and dendritic cells in the thymus. CD1a belongs to the CD1 family which presents lipid antigens to T cells. The protein is an important marker in immunophenotyping of these dendritic cells making it useful in flow cytometry applications.

Biological function summary

CD1a participates in the immune system by presenting lipid and glycolipid antigens to T lymphocytes including natural killer T (NKT) cells. It forms a complex with β2-microglobulin which is essential for its proper function and antigen presentation. CD1a expression helps differentiate between types of dendritic cells with CD1a-positive cells often engaging in specific responses to microbial lipid antigens.

Pathways

CD1a plays a significant role in the antigen presentation pathway particularly in lipid antigen presentation to T cells. This is important for initiating immune responses. It interacts with related proteins such as CD1b and CD1c which also present lipid antigens. CD1a has implications in innate and adaptive immunity and can influence pathways involved in microbial recognition and elimination.

CD1a is associated with certain skin conditions like Langerhans Cell Histiocytosis and psoriasis. It is involved in the pathogenesis of these diseases by affecting immune responses in the skin. In Langerhans Cell Histiocytosis CD1a-positive cells accumulate abnormally. Additionally CD1a is often studied alongside related proteins like CD207 (Langerin) to understand these conditions better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

See full target information CD1A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:25845-25864 PubMed33234721

2020

The long noncoding RNA LINC01140/miR-140-5p/FGF9 axis modulates bladder cancer cell aggressiveness and macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shuiqing Wu,Ran Xu,Xuan Zhu,Haiqing He,Jinhua Zhang,Qi Zeng,Yinhuai Wang,Xiaokun Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CD1a antibody [SK9]

Flow Cytometry - APC Anti-CD1a antibody [SK9] (AB234217)

Flow Cytometry - APC Anti-CD1a antibody [SK9] (AB234217)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise