AB134489

APC Anti-CD200 / OX2 antibody [OX104]

Name: Anti-CD200 / OX2 APC antibody [OX104] (ab134489) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB134489
Price: 305 USD
Availability: InStock
Rating: 3 (4 reviews)

(4 Reviews)

(2 Publications)

Mouse Monoclonal CD200 / OX2 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human, Cynomolgus monkey samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD200.

View Alternative Names

CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein

2 Images

Flow Cytometry - APC Anti-CD200 / OX2 antibody [OX104] (AB134489)

Flow Cyt

Unknown

Flow Cytometry - APC Anti-CD200 / OX2 antibody [OX104] (AB134489)

Flow cytometry analysis of normal Human peripheral blood cells with Anti-Human CD19 PE and 0.25 µg of Mouse IgG1 kappa Isotype Control APC (left) or 5 µl (0.25 µg) ab134489 (right). Cells in the lymphocyte gate were used for analysis.

Flow Cyt

AbReview33846****

Flow Cytometry - APC Anti-CD200 / OX2 antibody [OX104] (AB134489)

Flow cytometry analysis of Cynomolgus monkey whole blood after red blod cell lysis, staining CD200 / OX2 with ab134489. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C. .

Gating Strategy : Live lymphocytes

This image is courtesy of an anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX104

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human, Cynomolgus monkey

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD200. The exact immunogen used to generate this antibody is proprietary information.

P41217

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.

Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

See full target information CD200

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:13159 PubMed34162924

2021

Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Slaughter,John W Rumsey,Rachel Boone,Duaa Malik,Yunqing Cai,Narasimhan Narasimhan Sriram,Christopher J Long,Christopher W McAleer,Stephen Lambert,Michael L Shuler,J J Hickman

Stem cell research & therapy 10:155 PubMed31151466

2019

Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Huateng Zhou,Lixiang Wang,Cui Zhang,Jintao Hu,Jianlin Chen,Weibin Du,Fei Liu,Weifan Ren,Jinfu Wang,Renfu Quan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CD200 / OX2 antibody [OX104]

Flow Cytometry - APC Anti-CD200 / OX2 antibody [OX104] (AB134489)

Flow Cytometry - APC Anti-CD200 / OX2 antibody [OX104] (AB134489)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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