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AB25457

APC Anti-CD23 antibody [2G8]

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(1 Publication)

Rat Monoclonal CD23 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor

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Flow Cytometry - APC Anti-CD23 antibody [2G8] (AB25457)
  • Flow Cyt

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Flow Cytometry - APC Anti-CD23 antibody [2G8] (AB25457)

Flow cytometry analysis of BALB/c mouse splenocytes labeling CD23 with ab25457 at 0.1 μg/106cells and CD3 epsilon with Rat Anti-Mouse CD3 epsilon-FITC.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

2G8

Isotype

IgG2a

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab25457 recognises CD23 low affinity IgE Fc receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-apc-isotype-control-ab129719'>ab129719</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.
Biological function summary

CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

Pathways

CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells (PubMed : 2167225). On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed : 7544003).
See full target information FCER2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 180:4994-5003 PubMed18354225

2008

Suppression of proteoglycan-induced arthritis by anti-CD20 B Cell depletion therapy is mediated by reduction in autoantibodies and CD4+ T cell reactivity.

Applications

Flow Cyt

Species

Mouse

Keith Hamel,Paul Doodes,Yanxia Cao,Yumei Wang,Jeffrey Martinson,Robert Dunn,Marilyn R Kehry,Balint Farkas,Alison Finnegan
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