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AB242259

APC Anti-CD3 antibody [SK7]

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(1 Publication)

Mouse Monoclonal CD3d antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - APC Anti-CD3 antibody [SK7] (AB242259)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD3 antibody [SK7] (AB242259)

Flow Cytometric analysis of human peripheral blood lymphocyte cells labeling CD3 with 5 μl (0.5 μg) of ab242259 (solid line) or 0.5 μg APC-Cyanine7 Mouse IgG1 isotype control (Dashed line).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SK7

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>The antibody has been diluted for use at 5 μl per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl. The number of cells within a sample should be determined empirically, but typically ranges between 1 x 10e5 to 1 x 10e8 cells.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS via affinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 17:173 PubMed31881947

2019

The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yu,Xueshibojie Liu,Guanghong Han,Yan Liu,Xue Zhao,Di Wang,Xiaomin Bian,Tingting Gu,Lianji Wen
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Product promise

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