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Mouse Monoclonal CD3d antibody - conjugated to APC. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD3D protein.

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Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytFlow Cyt (Intra)
Human
Tested
Expected
Primates
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

10 µL for 106 Cells

Notes

(or 100 μl of whole blood).

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

10 µL for 106 Cells

Notes

(or 100 μl of whole blood).

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).

Alternative names

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Mouse Monoclonal CD3d antibody - conjugated to APC. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD3D protein.

Alternative names

Key facts

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
Clone number

UCHT1

Purification technique

Size-exclusion chromatography

Specificity

ab239287 reacts with the epsilon chain of the CD3 complex.

Concentration
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Purification notes

Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - APC Anti-CD3 antibody [UCHT1] (ab239287), expandable thumbnail

    Flow Cytometry - APC Anti-CD3 antibody [UCHT1] (ab239287)

    Flow cytometric analysis of human peripheral blood labeling CD3 with ab239287. Surface staining. Gated on leukocytes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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