AB210190

APC Anti-CD3 epsilon antibody [145-2C11]

Name: Anti-CD3 epsilon APC antibody [145-2C11] (ab210190) IgG | Abcam
Brand: Abcam Limited
SKU: AB210190

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(2 Publications)

Armenian hamster Monoclonal CD3E antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

1 Images

Flow Cytometry - APC Anti-CD3 epsilon antibody [145-2C11] (AB210190)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD3 epsilon antibody [145-2C11] (AB210190)

Flow cytometric analysis of mouse C57BI/6 splenocytes labeling CD3 with 0.25 μg ab210190 (solid line) compared with 0.25 μg armenian hamster IgG APC isotype control (dashed line).

Carrier free

Anti-CD3 epsilon antibody [145-2C11] - Low endotoxin, Azide free
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11]
675 PerCP

PerCP Anti-CD3 epsilon antibody [145-2C11]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

145-2C11

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab210190 is specific for mouse CD3e, also known as CD3 epsilon

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.25 μg.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Multi-step chromatography

Purification notes

ab210190 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Nature communications 9:4777 PubMed30429468

2018

MR imaging tracking of inflammation-activatable engineered neutrophils for targeted therapy of surgically treated glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Wu,Haixian Zhang,Changjun Tie,Chunhong Yan,Zhiting Deng,Qian Wan,Xin Liu,Fei Yan,Hairong Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CD3 epsilon antibody [145-2C11]

Flow Cytometry - APC Anti-CD3 epsilon antibody [145-2C11] (AB210190)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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