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AB325381

APC Anti-CD300a antibody [EPR27995-84]

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Rabbit Recombinant Monoclonal CD300a antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD300a, CMRF35H, IGSF12, HSPC083, CD300A, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CMRF35-H9, IgSF12, IRp60

3 Images
Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Cells were co-stained with anti human CD14 conjugated to Brilliant Violet 421.

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Cells were co-stained with anti human CD19 conjugated to PE/CY7.

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Negative control : 293T (PMID : 22043923)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27995-84

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed : 10746781, PubMed : 16339535, PubMed : 9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed : 22043923).
See full target information CD300A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com