Rat Monoclonal CD40 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD40.
Images
![Flow Cytometry - APC Anti-CD40 antibody [1C10] (AB25567), expandable thumbnail](https://content.abcam.com/products/images/apc-cd40-antibody-1c10-ab25567--flow-cytometry-img58656.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - APC Anti-CD40 antibody [1C10] (AB25567)")
Publications
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- Neuroscience letters 470:19-232009TNF-alpha receptor 1 deficiency reduces antigen-presenting capacity of Schwann cells and ameliorates experimental autoimmune neuritis in mice.Applications:Flow CytReactive species:MouseXi-Jing Mao,Xing-Mei Zhang,Hong-Liang Zhang,Hernan Concha Quezada,Eilhard Mix,Xin Yang,Bengt Winblad,Abdu Adem,Jie ZhuPubMed 20035831
Key facts
- Isotype
IgG2a
- Host species
Rat
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD40. The exact immunogen used to generate this antibody is proprietary information. Database link P25942
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
7 products for Alternative Product
Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD40, TNFRSF5
Recommended products
Rat Monoclonal CD40 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD40.
Key facts
- Isotype
IgG2a
- Host species
Rat
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD40. The exact immunogen used to generate this antibody is proprietary information. Database link P25942
- Clone number
1C10
- Purification technique
Affinity purification
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
This agonistic antibody closely resembles the gp39/CD40 ligand in it's ability to stimulate the proliferation of small, resting B lymphocytes in the absence of other cofactors. This antibody has been shown to be useful in studies of the 'in vitro' induction of proliferation of mature B lymphocytes.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - APC Anti-CD40 antibody [1C10] (ab25567), expandable thumbnail](https://content.abcam.com/products/images/apc-cd40-antibody-1c10-ab25567--flow-cytometry-img58656.jpg "Flow Cytometry - APC Anti-CD40 antibody [1C10] (ab25567)")
Flow Cytometry - APC Anti-CD40 antibody [1C10] (ab25567)
Flow cytometric analysis of BALB/c mouse splenocytes labeling CD40 using ab25567 at 0.1 μg/106 cells in 100 μL. CD3ε is labeled using a Rat Anti-Mouse CD3ε (FITC) antibody.
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