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AB210182

APC Anti-CD45 antibody [104]

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(4 Publications)

Mouse Monoclonal CD45 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

CD45, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

1 Images
Flow Cytometry - APC Anti-CD45 antibody [104] (AB210182)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD45 antibody [104] (AB210182)

Flow cytometric analysis of C57BI/6 splenocytes labeling CD45 with 0.5 μg ab210182 (solid line), compared with 0.5 μg of Allophycocyanin Mouse IgG2a isotype control (dashed line).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

104

Isotype

IgG2a

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.5 μg.</p>" } } }
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Product details

The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab210182 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.
See full target information Ptprc
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:6022-6043 PubMed40365283

2025

pH-responsive nano-vaccine combined with anti-PD-1 antibodies for enhanced immunotherapy of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Wang,Hong Yu,Jianqiao Yin,Xiaopeng Yu

iScience 27:110184 PubMed38974970

2024

Sirtuin 2 regulates neutrophil functions through NAD synthesis pathway in virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Zhang,Qiuli Yang,Yingjie Dong,Likun Wang,Ruiying Niu,Jingxuan Xia,Yujing Bi,Guangwei Liu

Stem cell research & therapy 12:235 PubMed33849640

2021

Bone marrow mesenchymal stem cell exosomes suppress phosphate-induced aortic calcification via SIRT6-HMGB1 deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Wei,Xiaodong Guo,Lijie Gu,Jieshuang Jia,Man Yang,Weijie Yuan,Shu Rong

Nature communications 8:153 PubMed28751718

2017

Two mouse models reveal an actionable PARP1 dependence in aggressive chronic lymphocytic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Gero Knittel,Tim Rehkämper,Darya Korovkina,Paul Liedgens,Christian Fritz,Alessandro Torgovnick,Yussor Al-Baldawi,Mona Al-Maarri,Yupeng Cun,Oleg Fedorchenko,Arina Riabinska,Filippo Beleggia,Phuong-Hien Nguyen,F Thomas Wunderlich,Monika Ortmann,Manuel Montesinos-Rongen,Eugen Tausch,Stephan Stilgenbauer,Lukas P Frenzel,Marco Herling,Carmen Herling,Jasmin Bahlo,Michael Hallek,Martin Peifer,Reinhard Buettner,Thorsten Persigehl,H Christian Reinhardt
View all publications
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