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AB28106

APC Anti-CD45 antibody [MEM-28]

5

(1 Review)

|

(10 Publications)

Mouse Monoclonal CD45 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

3 Images
Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (AB28106)
  • Flow Cyt

AbReview35307****

Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (AB28106)

ab28106 staining the CD45 in Human PBMCs by Flow Cytometry. Cells were prepared by Ficoll-Hypaque isolation of PBMCs from buffy coat. The sample was incubated with the primary antibody (1/25 in PBS + 2% Human serum and 1mM EDTA) for 25 minutes at 4°C.

This image is courtesy of an anonymous Abreview

Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (AB28106)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (AB28106)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28106 at (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (AB28106)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD45 antibody [MEM-28] (AB28106)

Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28106 (10 μl reagent / 100 μl of peripheral whole blood).

  • Unconjugated

    Anti-CD45 antibody [MEM-28]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD45 antibody [MEM-28]

  • Biotin

    Biotin Anti-CD45 antibody [MEM-28]

  • 675 PerCP

    PerCP Anti-CD45 antibody [MEM-28]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD45 antibody [MEM-28]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD45 antibody [MEM-28]

  • 618 PE/DyLight™ 594

    PE/DyLight™ 594 Anti-CD45 antibody [MEM-28]

  • 519 FITC

    FITC Anti-CD45 antibody [MEM-28]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD45 antibody [MEM-28]

  • 578 PE

    PE Anti-CD45 antibody [MEM-28]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-28

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

ab28106 reacts with all alternative forms of human CD45 antigen

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>For analysis of human blood cells use 10μl reagent / 100μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial is sufficient for 50 tests.</p>" } } }
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Product details

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 28:422-441 PubMed39918687

2025

Identification of CD66c as a potential target in gastroesophageal junction cancer for antibody-drug conjugate development.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Changjuan Tao,Hanfei Xie,Liu Yang,Ye Lu,Yun Xi,Shili Yao,Li Yuan,Peng Guo,Xiangdong Cheng

Biochemistry and biophysics reports 35:101497 PubMed37534324

2023

Umbilical mesenchymal stem cell-derived exosomes promote spinal cord functional recovery through the miR-146b/TLR4 -mediated NF-κB p65 signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiujuan Wang,Ying Yang,Wei Li,MingYuan Hao,YongSheng Xu

Methods in molecular biology (Clifton, N.J.) 2679:41-66 PubMed37300608

2023

Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization of Circulating Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammedin Deliorman,Ayoub Glia,Mohammad A Qasaimeh

STAR protocols 3:101433 PubMed35664257

2022

Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammedin Deliorman,Ayoub Glia,Mohammad A Qasaimeh

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:2646-2655 PubMed35112388

2022

Biomolecules-releasing click chemistry-based bioadhesives for repairing acetabular labrum tears.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxin Li,Cynthia M Co,Samira Izuagbe,Yi Hong,Jun Liao,Joseph Borrelli,Liping Tang

Bioactive materials 11:32-40 PubMed34938910

2021

Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haicong Shen,Rui Su,Jiao Peng,Lin Zhu,Kunyue Deng,Qi Niu,Yanling Song,Liu Yang,Lingling Wu,Zhi Zhu,Chaoyong Yang

Oncology letters 17:3799-3807 PubMed30881500

2019

Isolation of circulating tumor cells and detection of EGFR mutations in patients with non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Jingying Nong,Jinghui Wang,Zhuohong Yan,Ling Yi,Xin Gao,Zhidong Liu,Hongtao Zhang,Shucai Zhang

Biochimica et biophysica acta. Molecular cell rese 1865:695-708 PubMed29477577

2018

Similarities and differences between the responses induced in human phagocytes through activation of the medium chain fatty acid receptor GPR84 and the short chain fatty acid receptor FFA2R.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Sundqvist,Karin Christenson,André Holdfeldt,Michael Gabl,Jonas Mårtensson,Lena Björkman,Regis Dieckmann,Claes Dahlgren,Huamei Forsman

Oncotarget 8:103744-103757 PubMed29262597

2017

Membrane-bound full-length Sonic Hedgehog identifies cancer stem cells in human non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Etienne Giroux Leprieur,Bhairavi Tolani,Hui Li,Fleur Leguay,Ngoc T Hoang,Luis A Acevedo,Joy Q Jin,Hsin-Hui Tseng,Dongsheng Yue,Il-Jin Kim,Marie Wislez,Changli Wang,David M Jablons,Biao He

Folia biologica 34:23-34 PubMed2968928

1988

Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,P Angelisová,V Bazil,H Kristofová,S Stoyanov,I Stefanová,P Hausner,M Vosecký,I Hilgert
View all publications
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