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AB253088

APC Anti-CD49b antibody [HMa2]

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Armenian hamster Monoclonal Integrin alpha 2 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD49b, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa, Itga2

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Flow Cytometry - APC Anti-CD49b antibody [HMa2] (AB253088)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD49b antibody [HMa2] (AB253088)

Flow cytometric analysis of C57BI/6 splenocytes stained for CD49b using ab253088 at 0.125 μg (right panel) compared to 0.125 μg APC Armenian Hamster IgG isotype control (left panel).

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

HMa2

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use at 0.125 μg per reaction.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from Tissue culture supernatant.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD49b also known as Integrin alpha-2 or ITGA2 is a transmembrane glycoprotein with a molecular weight of approximately 160 kDa. This protein localizes on the surface of various cells including platelets fibroblasts and some immune cells. It functions as part of the integrin family which are receptors that mediate the cell-extracellular matrix (ECM) interactions. CD49b binds to collagen and laminin in the ECM and contributes to cellular adhesion and signal transduction.
Biological function summary

The CD49b protein plays a critical role in cell migration thrombosis and wound healing. By forming a heterodimer with Integrin beta-1 it becomes a part of the collagen receptor complex Integrin alpha-2/beta-1 which facilitates various cellular interactions with the ECM. This functionality enables cells to sense their environment and respond to mechanical changes which is important for processes such as tissue repair and embryonic development.

Pathways

Several cellular mechanisms involve CD49b in maintaining physiological homeostasis. One significant pathway is the integrin signaling pathway where CD49b interacts with focal adhesion kinase (FAK) and other intracellular proteins to regulate cytoskeletal rearrangement and cell motility. Another pathway is platelet activation where CD49b collaborates with glycoprotein VI (GPVI) and other receptors to initiate platelet aggregation during hemostasis. These pathways highlight the importance of CD49b in various cellular functions related to cell mobility and stability.

Researchers have linked CD49b to conditions including thrombosis and fibrosis. In thrombosis abnormal platelet aggregation often involves CD49b and its interaction with fibrinogen and von Willebrand factor. In fibrosis CD49b’s role in cell adhesion and matrix remodeling correlates with excessive ECM deposition and scar formation. Understanding CD49b’s function offers insights into therapeutic strategies alongside related proteins such as Integrin beta-1 which may modulate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-2/beta-1 is a collagen receptor, being responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides and E-cadherin. Mice homozygous for a null mutation in the alpha-2 die very early in embryogenesis.
See full target information Itga2
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