AB77025

APC Anti-CD52 antibody [HI186]

Name: APC Anti-CD52 antibody [HI186] (ab77025) | Abcam
Brand: Abcam Limited
SKU: AB77025

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Mouse Monoclonal CD52 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Tissue preparation containing CD52 protein.

View Alternative Names

CD52, CDW52, HE5, CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, He5

2 Images

Flow Cytometry - APC Anti-CD52 antibody [HI186] (AB77025)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD52 antibody [HI186] (AB77025)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab77025 (10 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD52 antibody [HI186] (AB77025)

Separation of human CD52 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab77025 (10 µl reagent / 100 µl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HI186

Isotype

IgG2b

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Tissue preparation containing CD52 protein. The exact immunogen used to generate this antibody is proprietary information.

P31358

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/apc-mouse-igg2b-plpv219-isotype-control-ab91534'>ab91534</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab77025 is purified by size exclusion chromatography and adjusted fordirect use.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD52 also known as Campath-1 antigen is a glycoprotein that weighs approximately 12 kDa. It is mainly expressed on the surface of lymphocytes monocytes macrophages and some epithelial cells. This protein is also present on T and B cells making it a reliable marker for these immune cells. CD52's role is primarily in cell adhesion and signaling. The anti-CD52 antibody alemtuzumab targets this antigen specifically indicating its utility in clinical applications.

Biological function summary

CD52 plays an essential role in immune response regulation. It is not part of a larger protein complex but interacts dynamically with cell membranes to mediate immune cell activity. By acting as a co-stimulatory molecule for T cells CD52 influences lymphocyte activation and proliferation. This activity becomes significant in modulating immune responses showcasing its importance in immunological pathways.

Pathways

CD52 heavily influences T cell receptor signaling and cytokine production pathways. It interacts with proteins in these pathways to facilitate effective immune surveillance and response. CD52's engagement in these pathways highlights its collaboration with proteins such as CD3 and CD28 which assist in T cell activation and adaptive immunity. Such interactions highlight CD52's role in maintaining immune homeostasis.

CD52 has substantial relevance in autoimmune diseases and hematological cancers. Multiple sclerosis is one such autoimmune disorder where aberrant CD52 activity affects disease progression. Alemtuzumab an anti-CD52 monoclonal antibody is often used in treatment emphasizing CD52's clinical significance. In hematological malignancies like chronic lymphocytic leukemia CD52 facilitates abnormal lymphocyte survival and proliferation. CD52's interplay with other cell surface proteins such as CD19 further delineates its role in disease pathology and targeting strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

See full target information CD52

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CD52 antibody [HI186]

Flow Cytometry - APC Anti-CD52 antibody [HI186] (AB77025)

Flow Cytometry - APC Anti-CD52 antibody [HI186] (AB77025)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise