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Rabbit Recombinant Monoclonal CD62L antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Target data

Function

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28489325, PubMed:28011641). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641).

Alternative names

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Rabbit Recombinant Monoclonal CD62L antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23565-109

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Activity summary

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

Associated diseases and disorders

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (ab317478), expandable thumbnail

    Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (ab317478)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with AB317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype contro.

    Cells were stained with anti-CD4 conjugated to Alexa Fluor® 488 and anti-CD45RO (BV421). Gated on viable CD4+ T cells.

  • Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (ab317478), expandable thumbnail

    Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (ab317478)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with AB317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Gated on viable cells.

  • Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (ab317478), expandable thumbnail

    Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (ab317478)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with AB317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Cells were stained with anti-CD4 conjugated to Alexa Fluor® 488. Gated on viable lymphocytes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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