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AB221251

APC Anti-CD68 antibody [FA-11]

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(4 Publications)

Rat Monoclonal CD68 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

CD68, Macrosialin, Cd68

1 Images
Flow Cytometry (Intracellular) - APC Anti-CD68 antibody [FA-11] (AB221251)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-CD68 antibody [FA-11] (AB221251)

Overlay histogram showing Raw264.7 cells stained with ab221251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221251, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in Raw264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

FA-11

Isotype

IgG2a

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab221251 detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information Cd68
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:12498-12509 PubMed38747738

2024

SHP2 mediates STAT3/STAT6 signaling pathway in TAM to inhibit proliferation and metastasis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guojing Chai,Yingbo Nan,Haili Zhao,Qingchuan Hu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2300509 PubMed37949677

2023

Ectopic Expression of a Truncated Isoform of Hair Keratin 81 in Breast Cancer Alters Biophysical Characteristics to Promote Metastatic Propensity.

Applications

Unspecified application

Species

Unspecified reactive species

Diane S Kang,Aidan Moriarty,Yiru Jess Wang,Amal Thomas,Jia Hao,Bret A Unger,Remi Klotz,Shamim Ahmmed,Yonatan Amzaleg,Stuart Martin,Siva Vanapalli,Ke Xu,Andrew Smith,Keyue Shen,Min Yu

Microbiology spectrum 11:e0225323 PubMed37796020

2023

Lactate promotes intracellular replication and systemic infection via driving macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Wang,Bin Yang,Shuangshuang Ma,Xiaolin Yan,Shuai Ma,Hongmin Sun,Yuyang Sun,Lingyan Jiang

Science advances 7: PubMed34039601

2021

Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Hu,Chenyu Chu,Li Liu,Chenbing Wang,Shue Jin,Renli Yang,Shengan Rung,Jidong Li,Yili Qu,Yi Man
View all publications
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Product promise

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