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AB326471

APC Anti-CD73 antibody [AD2]

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Mouse Monoclonal CD73 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

3 Images
Flow Cytometry - APC Anti-CD73 antibody [AD2] (AB326471)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD73 antibody [AD2] (AB326471)

Flow cytometry multicolor surface staining pattern of human lymphocytes using ab326471 (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) PE antibody(20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-CD73 antibody [AD2] (AB326471)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD73 antibody [AD2] (AB326471)

Separation of human CD73 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326471 (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-CD73 antibody [AD2] (AB326471)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD73 antibody [AD2] (AB326471)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326471 (10 μl reagent / 100 μl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD2

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5ґ-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed : 34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084).
See full target information NT5E
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Product promise

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