JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB95549

APC Anti-CD80 antibody [16-10A1]

Be the first to review this product! Submit a review

|

(2 Publications)

Armenian hamster Monoclonal CD80 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd80 protein.

View Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

1 Images
Flow Cytometry - APC Anti-CD80 antibody [16-10A1] (AB95549)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD80 antibody [16-10A1] (AB95549)

Flow cytometric analysis of C57Bl/6 splenocytes cells labeling CD80 with ab95549 at 0.06 μg (solid blue line) compared with APC Armenian Hamster isotype control at 0.06 μg (dashed black line). C57Bl/6 splenocytes were stimulated with anti-IgM and anti-CD40 for 4 days.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

16-10A1

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd80 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00609

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 mg/mL", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.142% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).
See full target information Cd80
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2207697 PubMed37740439

2023

In Situ Cocktail Nanovaccine for Cancer Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Mohan Liu,Daoyuan Xie,Die Hu,Rui Zhang,Yusi Wang,Lin Tang,Bailing Zhou,Binyan Zhao,Li Yang

Frontiers in microbiology 13:944006 PubMed35992649

2022

Locally generated C3 regulates the clearance of by IFN-γ-primed macrophage through regulation of xenophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liu,Yan Yan,Xiaoreng Wang,Nannan Chen,Jue Wu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB225674

Anti-CD80 antibody [EPR22183]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (AB225674)

  • 5
  • 1
Primary Antibodies

AB134120

Anti-CD80 antibody [EPR1157(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (AB134120)

  • 4
  • 2
Primary Antibodies

AB222702

Anti-CD80 antibody [EPR22127-251]

20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry - Anti-CD80 antibody [EPR22127-251] (AB222702)

  • 0
  • 0
Primary Antibodies

AB224797

Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (AB224797)

  • 0
  • 1
Primary Antibodies

AB238481

Anti-CD80 antibody [EPR22127-317]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CD80 antibody [EPR22127-317] (AB238481)

  • 0
  • 0

Complementary Products

Assay Kits

AB256392

Human CD80 ELISA Kit (B7-1)

Recombinant|SimpleStep

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

  • 0
  • 0
Primary Antibodies

AB275741

Alexa Fluor® 488 Anti-CD86 antibody [EPR22958-106]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [EPR22958-106] (AB275741)

  • 0
  • 0
Primary Antibodies

AB233046

Alexa Fluor® 647 Anti-CD11c antibody [N418]

Flow Cytometry - Alexa Fluor® 647 Anti-CD11c antibody [N418] (AB233046)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com