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AB27554

APC Anti-CD80 antibody [MEM-233]

4

(2 Reviews)

|

(3 Publications)

Mouse Monoclonal CD80 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD80.

View Alternative Names

CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7

  • Unconjugated

    Anti-CD80 antibody [MEM-233]

  • 519 FITC

    FITC Anti-CD80 antibody [MEM-233]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD80 antibody [MEM-233]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD80 antibody [MEM-233]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD80 antibody [MEM-233]

  • 578 PE

    PE Anti-CD80 antibody [MEM-233]

  • 675 PerCP

    PerCP Anti-CD80 antibody [MEM-233]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-233

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD80. The exact immunogen used to generate this antibody is proprietary information.

P33681

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10 uL antibody per 100 uL whole blood. <a href='/en-us/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
ab27554 was purified by size exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed : 10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed : 12121659). Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed : 36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD80
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 220: PubMed37796477

2023

TCR ligand potency differentially impacts PD-1 inhibitory effects on diverse signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Waipan Chan,Yuqi M Cao,Xiang Zhao,Edward C Schrom,Dongya Jia,Jian Song,Leah V Sibener,Shen Dong,Ricardo A Fernandes,Clinton J Bradfield,Margery Smelkinson,Juraj Kabat,Jyh Liang Hor,Grégoire Altan-Bonnet,K Christopher Garcia,Ronald N Germain

Scientific reports 7:810 PubMed28400599

2017

LPS promotes a monocyte phenotype permissive for human cytomegalovirus immediate-early gene expression upon infection but not reactivation from latency.

Applications

Flow Cyt

Species

Human

V G Kew,M R Wills,M B Reeves

PLoS pathogens 11:e1005001 PubMed26107716

2015

Recombinant Murine Gamma Herpesvirus 68 Carrying KSHV G Protein-Coupled Receptor Induces Angiogenic Lesions in Mice.

Applications

IHC

Species

Human

Junjie Zhang,Lining Zhu,Xiaolu Lu,Emily R Feldman,Lisa R Keyes,Yi Wang,Hui Fan,Hao Feng,Zanxian Xia,Jiya Sun,Taijiao Jiang,Shou-Jiang Gao,Scott A Tibbetts,Pinghui Feng
View all publications
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Product promise

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