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AB82392

APC Anti-CD9 antibody [EM-04]

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(1 Publication)

Rat Monoclonal CD9 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD9 antigen protein.

View Alternative Names

CD9, CD9 antigen, Cd9

1 Images
Flow Cytometry - APC Anti-CD9 antibody [EM-04] (AB82392)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD9 antibody [EM-04] (AB82392)

Surface staining of CD9 in murine peritoneal fluid cells with ab82392 4 μg/ml.

  • Unconjugated

    Anti-CD9 antibody [EM-04]

  • 578 PE

    PE Anti-CD9 antibody [EM-04]

  • 519 FITC

    FITC Anti-CD9 antibody [EM-04]

  • Biotin

    Biotin Anti-CD9 antibody [EM-04]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

EM-04

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD9 antigen protein. The exact immunogen used to generate this antibody is proprietary information.

P40240

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-3 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg1-kappa-monoclonal-klhg1-2-2-apc-isotype-control-ab154433'>ab154433</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Conjugate made from highly purified antibody (>95% by SDS-PAGE)
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed : 10634790, PubMed : 10634791, PubMed : 10700183, PubMed : 14715942). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (PubMed : 10634790, PubMed : 10634791, PubMed : 10700183, PubMed : 21690351). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (PubMed : 23575678). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (PubMed : 12796480). Acts as a receptor for PSG17 (PubMed : 11805154). Involved in platelet activation and aggregation (PubMed : 14715942). Regulates paranodal junction formation (PubMed : 14715942). Involved in cell adhesion, cell motility and tumor metastasis (By similarity). Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung (PubMed : 18662991).
See full target information CD9 antigen
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70091 PubMed40545975

2025

Extracellular Vesicles From Prostate Cancer-Corrupted Osteoclasts Drive a Chain Reaction of Inflammatory Osteolysis and Tumour Progression at the Bone Metastatic Site.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Tamura,Tomofumi Yamamoto,Akiko Kogure,Yusuke Yoshioka,Yusuke Yamamoto,Shinichi Sakamoto,Tomohiko Ichikawa,Takahiro Ochiya
View all publications
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