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Mouse Monoclonal CXCR4 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human, Primates samples. Immunogen corresponding to Cell preparation containing CXCR4 protein.

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Images

Flow Cytometry - APC Anti-CXCR4 antibody [12G5] (AB270635), expandable thumbnail
  • Flow Cytometry - APC Anti-CXCR4 antibody [12G5] (AB270635), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing CXCR4 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P61073

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Primates
Expected

Tested
Tested

Species
Human
Dilution info
10 µL for 106 Cells
Notes

-

Expected
Expected

Species
Primates
Dilution info
10 µL for 106 Cells
Notes

-

Target data

Function

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed:10452968, PubMed:18799424, PubMed:24912431, PubMed:28978524). Involved in the AKT signaling cascade (PubMed:24912431). Plays a role in regulation of cell migration, e.g. during wound healing (PubMed:28978524). Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels (PubMed:20228059). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival (By similarity). (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus (PubMed:10074122, PubMed:10756055, PubMed:8849450, PubMed:8929542, PubMed:9427609).

Alternative names

CD184, C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, LESTR, LAP-3, LPS-associated protein 3, SDF-1 receptor, CXCR4

Recommended products

Mouse Monoclonal CXCR4 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human, Primates samples. Immunogen corresponding to Cell preparation containing CXCR4 protein.

Key facts

Isotype
IgG2a
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing CXCR4 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P61073
Clone number
12G5
Purification technique
Size-exclusion chromatography
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCR4 also known as C-X-C chemokine receptor type 4 is a G protein-coupled receptor that is involved in signal transduction. It has a molecular weight of approximately 41 kDa. CXCR4 is ubiquitously expressed across various tissues including immune cells like T and B lymphocytes as well as in bone marrow brain and heart. It binds specifically with the ligand CXCL12 also known as stromal cell-derived factor 1 (SDF-1) facilitating responses such as cell migration and proliferation.

Biological function summary

CXCR4 plays an important role in the immune system hematopoiesis and angiogenesis. It does not function alone and is often part of a larger protein complex where it recruits and activates other G proteins. The receptor mediates chemotactic responses directing cells to sites of inflammation or injury. Its interaction with CXCL12 is critical for maintaining immune surveillance aiding in the movement and positioning of immune cells.

Pathways

CXCR4 integrates into significant cellular signaling pathways such as the PI3K/AKT pathway and the MAPK pathway. It collaborates closely with signaling proteins like AKT1 and MAPK1 impacting cell survival and growth. These pathways are essential for various cellular functions including cell cycle progression and apoptosis regulation. The cross-talk between CXCR4 and these pathways underlines its influence on cell fate decisions.

Associated diseases and disorders

CXCR4 is implicated in cancer metastasis and HIV entry into cells. Overexpression of CXCR4 is observed in several cancers contributing to tumor growth and metastasis. The interaction between CXCR4 and CXCL12 facilitates the infiltration and spread of cancer cells. Additionally in HIV CXCR4 serves as a coreceptor along with CD4 allowing the virus to enter and infect host cells. Both cancer and HIV illustrate CXCR4's central role in disease progression and pathogenesis.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - APC Anti-CXCR4 antibody [12G5] (ab270635), expandable thumbnail

    Flow Cytometry - APC Anti-CXCR4 antibody [12G5] (ab270635)

    Flow cytometric analysis of lymphocytes (red) and monocytes (black) cells labeling CD184 using ab270635. Concentration of 10 μl reagent/100μl peripheral whole blood.

  • Flow Cytometry - APC Anti-CXCR4 antibody [12G5] (ab270635), expandable thumbnail

    Flow Cytometry - APC Anti-CXCR4 antibody [12G5] (ab270635)

    Flow cytometric analysis of human peripheral whole blood cells labeling CD184 using ab270635, with the concentration of 10 μl reagent /100 μl of peripheral whole blood.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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