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Knockout Tested Rabbit Recombinant Monoclonal CXCR5 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - APC Anti-CXCR5 antibody [EPR23463-30] (AB316179), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/12500

Notes

-

Target data

Function

Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.

Alternative names

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CXCR5 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23463-30

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CXCR5 also known as CD185 is a chemokine receptor with a mass of approximately 43 kDa. It is part of the G protein-coupled receptor family and is widely expressed on follicular B helper T (Tfh) cells B cells and a subset of circulating blood cells. CXCR5 is recognized as a marker for Tfh cells which are critical for the function and maturation of B cells within germinal centers. In tissues CXCR5 expression appears prominently in regions where immune cell interactions facilitate immune and inflammatory responses.

Biological function summary

CXCR5 serves a significant role in directing B cells and Tfh cells to lymphoid tissues by interacting with its ligand CXCL13. This interaction involves recruitment and organization of B cells into follicles within secondary lymphoid organs. The presence of CXCR5 allows B cells and Tfh cells to navigate the follicular zones efficiently enhance antigen-specific immune responses and contribute to long-lived humoral immunity. CXCR5 functions as part of a complex that includes signaling pathways influencing cellular movements and location.

Pathways

CXCR5 plays a significant part in the immune system's trafficking pathways particularly within the crosstalk between chemokines and their receptors. CXCR5 expression facilitates the CXC chemokine pathway which important for positioning immune cells within lymphoid organs. Additionally CXCR5 interacts with proteins such as CXCL13 working together to pattern the architecture of germinal centers where affinity maturation and class switching of antibodies occur critical processes in adaptive immunity.

Associated diseases and disorders

CXCR5 has been implicated in autoimmune diseases and certain lymphomas. For example aberrant CXCR5 expression is linked to the pathogenesis of diseases like systemic lupus erythematosus (SLE) where it might contribute to immune dysregulation and chronic inflammation. In the context of certain lymphomas CXCR5's role in lymphoid organ architecture and cell migration becomes even more relevant. Its expression often parallels the presence of CXCL13 hinting at a network of interactions promoting tumor survival and growth within lymphoid landscapes. Understanding these relationships can offer insight into developing therapeutic strategies potentially involving CXCR5 inhibitors or anti-CXCR5 antibodies to address these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Flow Cytometry - APC Anti-CXCR5 antibody [EPR23463-30] (ab316179), expandable thumbnail

    Flow Cytometry - APC Anti-CXCR5 antibody [EPR23463-30] (ab316179)

    Flow cytometry overlay histogram showing wild-type Raji (green line) and CXCR5 knockout Raji stained with ab316179 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316179) (1x 106 in 100μl at 0.04 μg/ml (1/12500)) for 30min on ice.

    Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) was used at the same concentration and conditions as the primary antibody (wild-type Raji - black line, CXCR5 knockout Raji - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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