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AB270676

APC/Cy7® Anti-CD28 antibody [CD28.2]

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Mouse Monoclonal CD28 antibody - conjugated to APC/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD28 protein.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, TP44

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Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (AB270676)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (AB270676)

Flow cytometry analysis of human peripheral whole blood labeling CD28 using ab270676. Surface staining.

Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (AB270676)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (AB270676)

Flow cytometry analysis of human peripheral whole blood labeling CD28 positive lymphocytes using ab270676 (Red). Surface staining.

Control : Human neutrophil granulocytes (Black).

  • Carrier free

    Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free

  • 660 APC

    APC Anti-CD28 antibody [CD28.2]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD28 antibody [CD28.2]

  • Biotin

    Biotin Anti-CD28 antibody [CD28.2]

  • 519 FITC

    FITC Anti-CD28 antibody [CD28.2]

  • 578 PE

    PE Anti-CD28 antibody [CD28.2]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD28 antibody [CD28.2]

  • 675 PerCP

    PerCP Anti-CD28 antibody [CD28.2]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD28.2

Isotype

IgG1

Light chain type

kappa

Conjugation

APC/Cy7®

Excitation/Emission

Ex: 650nm, Em: 785nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.

P10747

Specificity

This antibody recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical co-stimulatory receptor of T cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in suspension.</p>" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 12196291, PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).
See full target information CD28
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