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AB210348

APC/Cy7® Anti-CD4 antibody [RM4-5]

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(4 Publications)

Rat Monoclonal CD4 antibody - conjugated to APC/Cy7®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images
Flow Cytometry - APC/Cy7® Anti-CD4 antibody [RM4-5] (AB210348)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC/Cy7® Anti-CD4 antibody [RM4-5] (AB210348)

Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.25 μg ab210348 (solid line), compared with 0.25 μg Allophycocyanin/Cy7® Rat IgG2a (dashed line).

  • 660 APC

    APC Anti-CD4 antibody [RM4-5]

  • 519 FITC

    FITC Anti-CD4 antibody [RM4-5]

  • 578 PE

    PE Anti-CD4 antibody [RM4-5]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD4 antibody [RM4-5]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD4 antibody [RM4-5]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD4 antibody [RM4-5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM4-5

Isotype

IgG2a

Light chain type

kappa

Conjugation

APC/Cy7®

Excitation/Emission

Ex: 650nm, Em: 785nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.25 μg.</p>" } } }
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Product details

The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will "compete" for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab210348 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Immunity 57:1665-1680.e7 PubMed38772365

2024

Metabolic coordination between skin epithelium and type 17 immunity sustains chronic skin inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ipsita Subudhi,Piotr Konieczny,Aleksandr Prystupa,Rochelle L Castillo,Erica Sze-Tu,Yue Xing,Daniel Rosenblum,Ilana Reznikov,Ikjot Sidhu,Cynthia Loomis,Catherine P Lu,Niroshana Anandasabapathy,Mayte Suárez-Fariñas,Johann E Gudjonsson,Aristotelis Tsirigos,Jose U Scher,Shruti Naik

Journal of controlled release : official journal of the Controlled Release Society 294:165-175 PubMed30557650

2018

Extracellular vesicles enhance the targeted delivery of immunogenic oncolytic adenovirus and paclitaxel in immunocompetent mice.

Applications

Unspecified application

Species

Unspecified reactive species

M Garofalo,A Villa,N Rizzi,L Kuryk,B Rinner,V Cerullo,M Yliperttula,V Mazzaferro,P Ciana

Viruses 10: PubMed30322158

2018

Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Mariangela Garofalo,Alessandro Villa,Nicoletta Rizzi,Lukasz Kuryk,Vincenzo Mazzaferro,Paolo Ciana
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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