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AB197130

APC/Cy7® Anti-CD45 antibody [MEM-28]

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(1 Publication)

Mouse Monoclonal CD45 antibody - conjugated to APC/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

2 Images
Flow Cytometry - APC/Cy7® Anti-CD45 antibody [MEM-28] (AB197130)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC/Cy7® Anti-CD45 antibody [MEM-28] (AB197130)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab197130 (concentration in sample 1.67 μg/ml).

Flow Cytometry - APC/Cy7® Anti-CD45 antibody [MEM-28] (AB197130)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC/Cy7® Anti-CD45 antibody [MEM-28] (AB197130)

Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab197130 (concentration in sample 1.67 μg/ml).

  • Unconjugated

    Anti-CD45 antibody [MEM-28]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD45 antibody [MEM-28]

  • 660 APC

    APC Anti-CD45 antibody [MEM-28]

  • Biotin

    Biotin Anti-CD45 antibody [MEM-28]

  • 675 PerCP

    PerCP Anti-CD45 antibody [MEM-28]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD45 antibody [MEM-28]

  • 618 PE/DyLight™ 594

    PE/DyLight™ 594 Anti-CD45 antibody [MEM-28]

  • 519 FITC

    FITC Anti-CD45 antibody [MEM-28]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD45 antibody [MEM-28]

  • 578 PE

    PE Anti-CD45 antibody [MEM-28]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-28

Isotype

IgG1

Conjugation

APC/Cy7®

Excitation/Emission

Ex: 650nm, Em: 785nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

ab197130 reacts with all alternative forms of Human CD45 antigen.

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Alternative Products

Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB221245

APC Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - APC Anti-CD45 antibody [EP322Y] (AB221245)

  • 0
  • 0
Primary Antibodies

AB40763

Anti-CD45 antibody [EP322Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] (AB40763)

  • 5
  • 14
Primary Antibodies

AB208022

Anti-CD45 antibody [EPR20033]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] (AB208022)

  • 5
  • 2
Primary Antibodies

AB229292

Anti-CD45 antibody [EPR20033] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (AB229292)

  • 5
  • 2
Primary Antibodies

AB214437

Anti-CD45 antibody [EP322Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] - BSA and Azide free (AB214437)

  • 5
  • 1

Complementary Products

Assay Kits

AB277445

Mouse CD45 ELISA kit

ELISA - Mouse CD45 ELISA kit (AB277445)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1
Primary Antibodies

AB314277

PE Anti-CD105 antibody [EPR22811-18]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD105 antibody [EPR22811-18] (AB314277)

  • 0
  • 0
Primary Antibodies

AB282789

PE Anti-CD73 antibody [EPR23048-139]

RabMAb|Recombinant

Flow Cytometry - PE Anti-CD73 antibody [EPR23048-139] (AB282789)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" }, "Horse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Computational and mathematical methods in medicine 2022:1986348 PubMed36092790

2022

GINS2 Is Downregulated in Peripheral Blood of Patients with Intervertebral Disk Degeneration and Promotes Proliferation and Migration of Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Jiang,Hailang Sun,Jian Dai,Cheng Zhang,Xiaoming Tang
View all publications
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Product promise

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