APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control Suitable for Flow Cyt and reacts with samples.
Images
![Flow Cytometry - APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control (AB239463), expandable thumbnail](https://content.abcam.com/products/images/apc-cy7-mouse-igg1-kappa-mopc-21-isotype-control-ab239463--flow-cytometry-img439015.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control (AB239463)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes ab239463 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
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Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Recommended products
APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control Suitable for Flow Cyt and reacts with samples.
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Clone number
MOPC-21
- Purification technique
Size-exclusion chromatography
- Specificity
ab239463 has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
- Light chain type
kappa
- Concentration
- Purification notes
Purified antibody is conjugated with tandem dye Allophycocyanin/Cy7 ® under optimum conditions. The reagent is free of unconjugated Allophycocyanin/Cy7 ®.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
1 product image
![Flow Cytometry - APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control (ab239463), expandable thumbnail](https://content.abcam.com/products/images/apc-cy7-mouse-igg1-kappa-mopc-21-isotype-control-ab239463--flow-cytometry-img439015.jpg "Flow Cytometry - APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control (ab239463)")
Flow Cytometry - APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control (ab239463)
Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) APC-Cy™7 antibody (concentration in sample 15 µg/ml).
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